Date: Tue, 08 May 2007 08:46:03 -0400 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(3L)BSC283 Cc: mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX Isolation and characterization of Df(3L)BSC283 Stacey Christensen, Jill Gresens and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC283 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08188 and PBac{WH}f01685. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; P{XP}d08188/PBac{WH}f01685 males. The males were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC283 from the segment of P{XP}d08188 to the left of its FRT site and the segment of PBac{WH}f01685 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC283 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 67C7;67D5. Df(3L)BSC283 failed to complement CG8108[EY14316] and Df(3L)AC1. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX