Date: Mon, 6 Sep 1999  15:16:48  +0200
To: Beverley Matthews <bmatthew@XXXX>
From: jcrbmc@XXXX (Jordi Casanova)
Subject: Re: point mutations in trk
>Dear Dr. Casanova,
>
>I am curator for FlyBase and am working on the annotation of the
>Drosophila genomic sequence. I am annotating the trk gene and am
>currently marking the sites of the mutations from your Oct. 1995 Genes
>and Development paper. The annotations will list your paper as the
>citation.
>
>There is a slight bit of ambiguity resulting from the fact that you
>list the mutations in terms of amino acid changes and not nucleic acid
>changes. I can't quite pinpoint the nucleic acid site of the mutations
>for trk[3] or trk[1]. The TGG codons could have changed to either TGA
>or TAG to give the stop codons in the mutants. Can you tell me what the
>nucleic acid changes were so I can make the appropriate annotations?
>Can you also confirm that the trk[6] change is a G to A change (amino
>acid change R67Q) and the trk[2] mutation is a G to C change (amino
>acid change C169S)? For the latter, it says C168S in the paper but I
>believe the change is in codon 169.
>
>Thanks for your help.
>
>Sincerely,
>
>Beverley Matthews
>Curator for FlyBase
Please, find enclosed the data you requested:
trk1 TGG (trp) changed to TGA (stop)
trk2 TGC (cys) changed to AGC (ser)
trk3 TGG (trp) changed to TAG (stop)
trk6 CGA (arg) changed to CAA (gln)
Sincerely,
Jordi Casanova