Date: Wed, 08 Mar 2006 13:13:03 \-0500 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(2L)BSC143 Cc: Stacey Christensen <sjchristXXXX>, mdealXXXX, Jill Gresens <jgresens@XXXX> Isolation and characterization of Df(2L)BSC143 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2L)BSC143 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG5731f00094 and P{XP}d05337a. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG5731f00094/P{XP}d05337a males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC143 from the segment of PBac{WH}CG5731f00094 to the left of its FRT site and the segment of P{XP}d05337a to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC143 predicted from the transposable element insertions sites using Release 3 coordinates are 31B1;31D9. It failed to complement chico1, trk1, and bsk1. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX
