From kcook@XXXX Thu Sep 11  19:40:31  2003
To: rd120@XXXX
Subject: Myster and Peifer lethals
The following information accompanied stocks donated to the Bloomington
Stock Center by Steve Myster and Mark Peifer, University of North Carolina
(6/03).
1. A cn1 bw1 chromosome was mutagenized with EMS and lethal mutations
were isolated that failed to complement Df(2R)M41A8. This led to the
identification of the following complementation groups:
l(2)NC83 1 allele = l(2)NC83NC83
l(2)NC30 1 allele = l(2)NC30NC30
l(2)NC28 1 allele = l(2)NC28NC28
l(2)NC37 1 allele = l(2)NC37NC37
l(2)NC38 2 alleles = l(2)NC38NC38 and l(2)NC38NC173
l(2)NC133 1 allele = l(2)NC133NC133
l(2)NC110 1 allele = l(2)NC110NC110
l(2)NC70 1 allele = l(2)NC70NC70
l(2)NC19 6 alleles including l(2)NC19NC19 and l(2)NC19NC86
l(2)NC204 1 allele = l(2)NC204NC204
l(2)NC192 1 allele = l(2)NC192NC192
l(2)NC85 2 alleles including l(2)NC85NC85 and l(2)NC85NC117
l(2)NC148 1 allele = l(2)NC148NC148
l(2)NC139 1 allele = l(2)NC139NC139
l(2)NC45 1 allele = l(2)NC45NC45
l(2)NC130 6 alleles including l(2)NC130NC130 and l(2)NC130NC95
l(2)NC12 1 allele = l(2)NC12NC12
l(2)NC47 3 alleles including l(2)NC47NC47 and l(2)NC47NC84
l(2)NC114 1 allele = l(2)NC114NC114
l(2)NC14 4 alleles including l(2)NC14NC14 and l(2)NC14NC54
l(2)NC136 2 alleles including l(2)NC136NC136 which failed to
complement P{SUPor-P}CG8426KG10496
2. Additional alleles of the following complementation groups were isolated:
l(2)IR3 5 new alleles including l(2)IR3NC1
l(2)41Af 1 new allele called l(2)41AfNC21
Nipped-A 36 new alleles including Nipped-ANC116 and Nipped-ANC186
3. The following deletions were isolated from the screen:
Df(2R)NC138 which failed to complement mutations in l(2)NC30 and l(2)NC28
Df(2R)NC109 which failed to complement mutations in l(2)NC37 and l(2)NC38
Df(2R)NC9 which failed to complement mutations in l(2)IR3, l(2)IR23 and
l(2)NC19
Df(2R)NC89 which failed to complement mutations in l(2)IR23 and l(2)NC19
Df(2R)NC13 and Df(2R)NC228 which failed to complement mutations in
l(2)NC83, l(2)NC30, l(2)NC28, l(2)309, l(2)NC37, l(2)NC38, l(2)NC133,
l(2)NC110, l(2)NC70, l(2)IR3, l(2)IR23, l(2)NC19, l(2)41Af, l(2)NC204,
Nipped-B, Nipped-A, l(2)NC192, l(2)NC85, l(2)NC148, l(2)NC139, l(2)NC45,
l(2)NC130, l(2)NC12, l(2)NC47, l(2)NC114, l(2)NC14, l(2)NC136, vlc and Bub1
and deleted the CG40293, p120ctn and CG17486 sequences.
Df(2R)NC73 which failed to complement mutations in l(2)NC192, l(2)NC85,
l(2)NC148, l(2)NC139, l(2)NC45, l(2)NC130, l(2)NC12, l(2)NC47, l(2)NC114,
l(2)NC14, l(2)NC136 and vlc.
Df(2R)NC32 which failed to complement mutations in l(2)NC130, l(2)NC12 and
l(2)NC47
Df(2R)NC219 which failed to complement mutations in l(2)NC47, l(2)NC114 and
l(2)NC14
Df(2R)NC207 which failed to complement mutations in l(2)NC14 and l(2)NC136
4. Df(2R)Dark2, Df(2R)244, Df(2R)247 and P{SUPor-P}l(2)3091 were
isolated after mobilization of P{SUPor-P}KG01086. Df(2R)Dark2 deletes the
CG17486 sequence. Df(2R)244 deletes the CG40293 and p120ctn
sequences. Df(2R)247 fails to complement mutations in l(2)NC37, l(2)NC38,
l(2)NC133, l(2)NC110, l(2)NC70, l(2)IR3, l(2)IR23, l(2)NC19, l(2)41Af and
l(2)NC204 and deletes the CG40293 and p120ctn sequences.
5. Hilliker (Genetics 83: 765-782, 1976; FBrf0028786) described l(2)41Ae
as a complex locus showing complicated interallelic complementation
patterns. Some or all of the mutations defining the twelve most proximal
complementation groups listed above (l(2)NC83, l(2)NC30, l(2)NC28, l(2)309,
l(2)NC37, l(2)NC38, l(2)NC133, l(2)NC110, l(2)NC70, l(2)IR3, l(2)IR23 and
l(2)NC19) may be alleles of l(2)41Ae
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-855-5782
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX