Hoskins, R.A., Nelson, C.R., Berman, B.P., Laverty, T.R., George, R.A., Ciesiolka, L., Naeemuddin, M., Arenson, A.D., Durbin, J., David, R.G., Tabor, P.E., Bailey, M.R., De Shazo, D.R., Catanese, J., Mammoser, A., Osoegawa, K., de Jong, P.J., Celniker, S.E., Gibbs, R.A., Rubin, G.M., Scherer, S.E. (2000). A BAC-based physical map of the major autosomes of Drosophila melanogaster.  Science 287(5461): 2271--2274.

FBrf0127123

Research paper

We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.