FB2024_04 , released June 25, 2024
Reagent: RPCI-98_BAC
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General Information
Name
RPCI-98_BAC
Species
D. melanogaster
Reagent type
genomic clone collection
FlyBase ID
FBlc0000777
Project
Created by
BDGP
Vector
    Title
    BAC genomic clone collection for D. melanogaster, iso-1 strain, adult, 163kb inserts.
    Accessions
    Overview
    Description

    A BAC library containing genomic DNA fragments with average size of 163 kb. Generated for physical mapping of the Drosophila genome.

    (Hoskins et al., 2000)
    Available from
    Biosample Source
    Overview
    Strain
    iso-1
    (Hoskins et al., 2000)
    Stage
    adult stage
    (Hoskins et al., 2000)
    Sex
    Tissue isolated
    organism
    (Hoskins et al., 2000)
    Other tissues studied
    Cell component
    nucleus
    (Hoskins et al., 2000)
    Cell line
    Key genes
    Methods
    Sample preparation
    Biosamples used (1)
    Biosample
    Type
    Title
    BS_RPCI-98_BAC
    whole organism
    D. melanogaster, iso-1 strain, adult, nucleus, source for BAC genomic clones.
    Reagent Details
    Methods
    size fractionation
    (Hoskins et al., 2000)
    Protocol

    DNA was isolated from adult fly tissue and embedded in agarose blocks. DNA was partially digested with EcoRI and EcoRI methylase, size fractionated and cloned into the pBACe3.6 vector.

    (Hoskins et al., 2000)
    Mode of Assay
    Data analysis
    Comments

    A total of 17,540 recombinant clones were initially generated with an average insert size of 163kb (24-fold coverage of the euchromatic portion of the genome)

    (Hoskins et al., 2000)
    Associated Data
    Size
    Associated features
    962 Clone(s)
    Download 
    Files
    Additional Information
    Synonyms and Secondary IDs (5)
    Reported As
    Symbol Synonym
    BACR
    BDGP BAC library
    RPCI-98
    (Hoskins et al., 2000)
    RPCI-98_BAC
    Name Synonyms
    BAC genomic clone collection for D. melanogaster, iso-1 strain, adult, 163kb inserts.
    (Hoskins et al., 2000)
    Secondary FlyBase IDs
      References (2)