FB2024_02 , released April 23, 2024
Project: Furlong_BiTS-ChIP-Seq
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General Information
Name
Furlong_BiTS-ChIP-Seq
Species
D. melanogaster
Project type
genome binding
FlyBase ID
FBlc0003341
Parent Project
    Data Provider
    Title
    ChIP-Seq profiling of histone modifications in purified embryonic mesodermal cells.
    Accessions
    Overview
    Description
    Project attributes
    Biosample type
    isolated cells
    (Bonn et al., 2012)
    whole organism
    (Bonn et al., 2012)
    Assay type
    ChIP-Seq
    (Bonn et al., 2012)
    Reagent type
    Result type
    binding site identification
    (Bonn et al., 2012)
    Key genes
    (bait_protein)
    GO term(s)
    SO term(s)
    TF_binding_site
    region
    Details
    Sample preparation
    Protocol

    Chromatin was prepared from formaldehyde-fixed embryos as previously described (FBrf0202481).

    (Monfort and Furlong, 2015.1.15)
    Mode of Assay

    Chromatin immunoprecipitated DNA was analyzed by next generation sequencing.

    (Monfort and Furlong, 2015.1.15)
    Data analysis

    Reads were aligned using bowtie. Input DNA or His3 ChIP DNA was used as control data. Peaks were called with MACS 1.3.7.1. Peak calls were lifted over from Dmel_Release_5 to Dmel_Release_6 using the NCBI Genome Remapping tool.

    (Monfort and Furlong, 2015.1.15)
    Comments
    Files
    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    Furlong_BiTS-ChIP-Seq
    Name Synonyms
    ChIP-Seq profiling of histone modifications in purified embryonic mesodermal cells.
    (Monfort and Furlong, 2015.1.15)
    Secondary FlyBase IDs
      References (3)