FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
aub, csul
FlyBase ID
FBig0000121182
Interaction Network
Interactions Browser links
aub network
csul network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0249362-11.coIP.WB
Reference
Huang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
csul 

Csul

bait

FLAG tag

aub 

Aub

prey

HA tag

aub 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
piRNA binding domain
mutation disrupting interaction
aa 568,572

Y568L,K572E, coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Loading aub with synthetic RNA increases the auc-csul interaction.

(Huang et al., 2021)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2021)
FBrf0249362-9.coIP.WB
Reference
Huang et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
aub 

Aub

bait

FLAG tag

aub 
csul 

Csul

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aub 
N-terminal domain
sufficient binding region
aa 1-220

coordinates relative to aub-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Huang et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)