Source was a transgenic fly line homozygous for a point mutation in vls leading to a premature stop codon; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
Source was a transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
six independent interacting clones of which four of them corresponded to the CG10728 coding sequence which codes for vls
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
