FB2024_04 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Abi, Cdk1
FlyBase ID
FBig0000108641
Interaction Network
Interactions Browser links
Abi network
Cdk1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0184055-1.Y2H
Reference
Lin et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abi 

dAbi

bait

fused to GAL4 DNA-binding domain

Cdk1 

dCdc2

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Lin et al., 2004)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Lin et al., 2004)
FBrf0184055-2.Y2H
Reference
Lin et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abi 

dAbi

bait

fused to LexA DNA-binding domain

Cdk1 

dCdc2

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Lin et al., 2004)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Lin et al., 2004)
FBrf0184055-3.PD.WB
Reference
Lin et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

Cdc2

bait

GST tag

Abi 

Abi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lin et al., 2004)
FBrf0184055-4.PD.WB
Reference
Lin et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait

GST tag

Cdk1 

Cdc2

prey

HA tag

Cdk1 
Cdk1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cdk1 
N-terminal region
sufficient binding region
aa 1-208

coordinates relative to Cdk1-PA

Cdk1 
N-terminal region
necessary binding region
aa 7-181

coordinates relative to Cdk1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lin et al., 2004)

Abi pulls down Cdk1 but not Cdk2 or Cdk5 in this assay.

(Lin et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)