FB2024_04 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Abi, Cip4
FlyBase ID
FBig0000103781
Interaction Network
Interactions Browser links
Abi network
Cip4 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0209286-7.Y2H
Reference
Fricke et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait

fused to GAL4 DNA-binding domain

Cip4 

Cip4

prey

fused to GAL4 activation domain

Cip4 
Cip4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cip4 
SH3 domain
sufficient binding region
Cip4 
SH3 domain
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fricke et al., 2009)

Cip4 binds WASp and Abi, but not WAVE, in a two hybrid assay.

(Fricke et al., 2009)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Fricke et al., 2009)
FBrf0209286-8.coIP.WB
Reference
Fricke et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Abi 

Abi

bait
Cip4 

Cip4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Fricke et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)