FB2024_04 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
fwe
FlyBase ID
FBig0000094921
Interaction Network
Interactions Browser links
fwe network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0209490-2.coIP.WB
Reference
Yao et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fwe 

Flower

bait

HA tag

fwe 

Flower

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was animals (stage not specified) from a transgenic line homozygous for a mutation in the endogenous fwe gene and carrying tagged fwe constructs.

(Yao et al., 2009)
FBrf0209490-3.coIP.WB
Reference
Yao et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fwe 

Flower

bait

GFP tag

fwe 

Flower

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was animals (stage not specified) from a transgenic line homozygous for a mutation in the endogenous fwe gene and carrying tagged fwe constructs.

(Yao et al., 2009)
FBrf0209490-4.coIP.WB
Reference
Yao et al., 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fwe 

Flower

self

HA tag

fwe 

Flower

self

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was animals (stage not specified) from a transgenic line homozygous for a mutation in the endogenous fwe gene and carrying tagged fwe constructs.

(Yao et al., 2009)

fwe can form higher order multimers.

(Yao et al., 2009)

HA-tagged fwe was immunoprecipitated, eluted by HA peptide, fractionated by non-denaturing gel electrophoresis and detected by western blot.

(Yao et al., 2009)
FBrf0253239-7.coIP.WB
Reference
Merino et al., 2022
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fwe 

fwe

bait

GFP tag

fwe 

fwe

prey

RFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Extended data Fig3C

(Merino et al., 2022)

Source was wing discs of transgenic fly line; bait and prey produced from tagged transgenic constructs.

(Merino et al., 2022)

fwe contains YFP, GFP and RFP tags

(Merino et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (2)