FlyBase Gene Report: Dmel\CG33346

SINGLE-STRANDED DNA ENDODEOXYRIBONUCLEASES -: Single-stranded DNA endodeoxyribonucleases catalyze the hydrolysis of ester linkages within a single-stranded deoxyribonucleic acid molecule by creating internal breaks.
UNCLASSIFIED RNA ENDONUCLEASES -: This group comprises endoribonucleases that do not classify under other groups in FlyBase.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG33346 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KRX0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Mutation in sequenced strain: premature stop (nonsense mutation).

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG33346-RB

FBtr0302527

NM_206578

1096

364

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG33346-PB

FBpp0291689

41.4

364

9.06

Q7KRX0

NP_996301

AAS65221

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q7KRX0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG33346 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG33346_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.34

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG33346 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG33346

View exonic expression by tissue for Dmel\CG33346

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

2768690/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053346

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053346

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053346

Flygut - An atlas of the Drosophila adult midgut

FBgn0053346

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG33346

Transgenic constructs containing regulatory region of CG33346

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG33346^GD16071

viable, with Scer\GAL4^Mef2.PR

CG33346^GD16071

viable, with Scer\GAL4^pnr-MD237

CG33346^GD16071

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CG33346^GD16071

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (4)

Hsap\EXOG

4 of 14

Yes

Hsap\ENDOG

2 of 14

Hsap\ENPP1

1 of 14

Yes

Hsap\ENPP2

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (3)

Rnor\Exog

4 of 14

Yes

Rnor\Endog

2 of 14

Rnor\Enpp2

1 of 14

Yes

Mus musculus (laboratory mouse) (3)

Mmus\Exog

4 of 14

Yes

Mmus\Endog

1 of 14

Mmus\Enpp2

1 of 14

Xenopus tropicalis (Western clawed frog) (10)

Xtro\endog

2 of 13

Yes

Xtro\LOC100487990

2 of 13

Yes

Xtro\LOC100490758

2 of 13

Yes

Xtro\LOC101731854

2 of 13

Yes

Xtro\XB5812267

2 of 13

Yes

Xtro\exog

1 of 13

Yes

Xtro\LOC105945145

1 of 13

Yes

Xtro\LOC116409498

1 of 13

Yes

Xtro\XB5738616

1 of 13

Xtro\XB5856157

1 of 13

Yes

Danio rerio (Zebrafish) (20)

Drer\si:dkey-243k1.3

3 of 14

Yes

Drer\endog

2 of 14

Drer\LOC100002544

2 of 14

Yes

Drer\LOC101885869

2 of 14

Yes

Drer\si:ch211-133n4.4

2 of 14

Yes

Drer\si:ch211-133n4.9

2 of 14

Yes

Drer\si:ch211-165i18.2

2 of 14

Yes

Drer\si:dkey-85k7.11

2 of 14

Yes

Drer\zgc:172339

2 of 14

Yes

Drer\enpp2

1 of 14

Drer\LOC100333474

1 of 14

Yes

Drer\LOC101883376

1 of 14

Yes

Drer\LOC101885345

1 of 14

Yes

Drer\LOC101886621

1 of 14

Yes

Drer\LOC103910196

1 of 14

Yes

Drer\LOC103911895

1 of 14

Yes

Drer\si:dkey-85k7.10

1 of 14

Drer\wu:fd46g04

1 of 14

Yes

Drer\zgc:153118

1 of 14

Yes

Drer\zgc:158445

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\cps-6

2 of 14

Yes

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP011698

3 of 12

Yes

Agam\AgaP_AGAP004760

2 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP011046

1 of 12

Agam\AgaP_AGAP011047

1 of 12

Agam\AgaP_AGAP011048

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\NUC1

2 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\pnu1

2 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG33346. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (12)

CG14062

7 of 13

Sid

7 of 13

6 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EXOG; exo/endonuclease G

4 of 14

604051

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0053346

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-97

Cytogenetic map

98E1-98E1

Sequence location

3R:28,669,233..28,670,452 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

98E1-98E1

Limits computationally determined from genome sequence between P{PZ}l(3)06487⁰⁶⁴⁸⁷ and P{EP}EP3390^EP3390 98E1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-97

(FlyBase, 2016)

3-93.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

33214

y¹ w^*; Mi{MIC}CG33346^MI02377/TM3, Sb¹ Ser¹

VDRC

v100352

P{KK106030}VIE-260B

v47842

w¹¹¹⁸; P{GD16071}v47842

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

IP21418

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0053346

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0053346

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG33346) in release 3.2 of the genome annotation.

(FlyBase Genome Annotators, 2004)

Probable pseudogene.

(FlyBase Genome Annotators, 2004)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

CG33346

(Balakireva et al., 2024, Muita and Baxter, 2023, Orthofer et al., 2020, Mossman et al., 2016, Huang et al., 2013, Levine et al., 2011, Fernández-Ayala et al., 2010, Keleman et al., 2009.8.5, Adryan et al., 2007)

Name Synonyms

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 26 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

2768690

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAS65221

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KRX0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q7KRX0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KRX0

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

2768690/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0053346

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0053346

FlyMine - An integrated database for Drosophila genomics

FBgn0053346

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33346

MARRVEL_MODEL - MARRVEL (model organism gene)

2768690

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0053346

DroID - A comprehensive database of gene and protein interactions.

FBgn0053346

DRSC - Results frm RNAi screens

FBgn0053346

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG33346_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0053346

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0053346

Flygut - An atlas of the Drosophila adult midgut

FBgn0053346

References (27)

The following external sites may use different assemblies or annotations than FlyBase.

-0.34

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