FB2024_02 , released April 23, 2024
Gene: Dmel\mt:ATPase6
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General Information
Symbol
Dmel\mt:ATPase6
Species
D. melanogaster
Name
mitochondrial ATPase subunit 6
Annotation Symbol
CG34073
Feature Type
protein_coding_gene
FlyBase ID
FBgn0013672
Gene Model Status
Current
Stock Availability
4 publicly available
Enzyme Name (EC)
H(+)-transporting two-sector ATPase (7.1.2.2)
Gene Summary
mitochondrial ATPase subunit 6 (mt:ATPase6) encodes a mitochondrial inner transmembrane protein that is key for oxidative phosphorylation. It couples hydrogen ion transport to ATP synthesis functioning as part of the ATP synthase. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

ATP6, ATPase6, mtATP6, mt:ATP6 , ATPase 6

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
RefSeq locus
NC_024511 REGION:4062..4736
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
Function
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables proton transmembrane transporter activity
inferred from electronic annotation with InterPro:IPR000568, InterPro:IPR045083
(InterPro Project Members, 2004-)
contributes_to proton-transporting ATP synthase activity, rotational mechanism
inferred from biological aspect of ancestor with PANTHER:PTN000151034
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:P00854
(Gene Ontology Curators, 2002-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in determination of adult lifespan
inferred from mutant phenotype
(Celotto et al., 2006)
involved_in locomotion
inferred from mutant phenotype
(Celotto et al., 2006)
involved_in muscle cell cellular homeostasis
inferred from mutant phenotype
(Celotto et al., 2006)
involved_in neuron cellular homeostasis
inferred from mutant phenotype
(Celotto et al., 2006)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in proton motive force-driven ATP synthesis
inferred from sequence or structural similarity with UniProtKB:P00854
(Gene Ontology Curators, 2002-)
inferred from biological aspect of ancestor with PANTHER:PTN000151034
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR000568, InterPro:IPR045083
(InterPro Project Members, 2004-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in mitochondrion
inferred from mutant phenotype
(Celotto et al., 2006)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
part_of mitochondrial proton-transporting ATP synthase complex, coupling factor F(o)
inferred from sequence or structural similarity with UniProtKB:P00854
(Gene Ontology Curators, 2002-)
part_of mitochondrial proton-transporting ATP synthase complex
inferred from biological aspect of ancestor with PANTHER:PTN000151035
(GO Reference Genome Project, 2011-)
part_of proton-transporting ATP synthase complex, coupling factor F(o)
inferred from electronic annotation with InterPro:IPR000568, InterPro:IPR045083
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the ATPase A chain family. (P00850)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
proton-transporting ATP synthase activity, rotational mechanism
ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate (7.1.2.2)
RHEA 57722: RHEA 57720:
Summaries
Gene Snapshot
mitochondrial ATPase subunit 6 (mt:ATPase6) encodes a mitochondrial inner transmembrane protein that is key for oxidative phosphorylation. It couples hydrogen ion transport to ATP synthesis functioning as part of the ATP synthase. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
MITOCHONDRIAL COMPLEX V - F0 SUBUNITS -
Mitochondrial ATP synthase (Complex V) synthesizes ATP from ADP and inorganic phosphate using the energy provided by the proton electrochemical gradient across the inner mitochondrial membrane. It consists of two structural domains: the F1 sector, a soluble portion situated in the mitochondrial matrix, and the F0 sector, which spans the inner mitochondrial membrane. The F0 sector consists of an integral membrane proton-conducting channel and a peripheral stalk domain. (Adapted from PMID:21874297).
MITOCHONDRIAL COMPLEX V - TESTIS-SPECIFIC VARIANT -
Mitochondrial ATP synthase (Complex V) synthesizes ATP from ADP and inorganic phosphate using the energy provided by the proton electrochemical gradient (proton-motive force) across the inner mitochondrial membrane generated by electron transport complexes of the respiratory chain. It is an F-type ATPase and consists of two structural domains: the F1 sector, a soluble portion situated in the mitochondrial matrix, and the F0 sector, which spans the inner mitochondrial membrane. Protons pass from the inter membrane space to the matrix through F0, which transfers the energy created by the proton-motive force to F1 where ADP is phosphorylated to ATP using a rotational mechanism. (Adapted from PMID:23356252 and PMID:21874297).
MITOCHONDRIAL COMPLEX V -
Mitochondrial ATP synthase (Complex V) synthesizes ATP from ADP and inorganic phosphate using the energy provided by the proton electrochemical gradient (proton-motive force) across the inner mitochondrial membrane generated by electron transport complexes of the respiratory chain. It is an F-type ATPase and consists of two structural domains: the F1 sector, a soluble portion situated in the mitochondrial matrix, and the F0 sector, which spans the inner mitochondrial membrane. Protons pass from the inter membrane space to the matrix through F0, which transfers the energy created by the proton-motive force to F1 where ADP is phosphorylated to ATP using a rotational mechanism. (Adapted from PMID:23356252 and PMID:21874297).
Protein Function (UniProtKB)
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane.
(UniProt, P00850)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\mt:ATPase6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P00850)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model
Sequence Ontology: Class of Gene
mt_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mt:ATPase6-RB
FBtr0433498
675
224
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
mt:ATPase6-PB
FBpp0390630
25.2
224
7.70
P00850
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

(UniProt, P00850)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mt:ATPase6 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in mitochondrion
inferred from mutant phenotype
(Celotto et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mt:ATPase6 in JBrowse
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mt:ATPase6
Transgenic constructs containing regulatory region of mt:ATPase6
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
    Variants
    Variant Molecular Consequences
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Human Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Homo sapiens (Human) (1)
    Hsap\ATP6
    12 of 14
    Yes
    Yes
    Model Organism Orthologs (via DIOPT v9.1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    Domainoid
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    SonicParanoid
    Alignment
    Complementation?
    Transgene?
    Rattus norvegicus (Norway rat) (1)
    Rnor\ATP6
    12 of 14
    Yes
    Yes
    Mus musculus (laboratory mouse) (2)
    Mmus\ATP6
    11 of 14
    Yes
    Yes
    Mmus\Gm10925
    10 of 14
    No
    Yes
    Xenopus tropicalis (Western clawed frog) (1)
    Xtro\ATP6
    10 of 13
    Yes
    Yes
    Danio rerio (Zebrafish) (1)
    Drer\ATP6
    12 of 14
    Yes
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (1)
    Cele\ATP6
    8 of 14
    Yes
    Yes
    Anopheles gambiae (African malaria mosquito) (1)
    Agam\ATP6
    10 of 12
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (4)
    Atha\AT2G07741
    9 of 13
    Yes
    Yes
    Atha\atp6
    8 of 13
    No
    Yes
    Atha\atp6
    8 of 13
    No
    Yes
    Atha\atpI
    4 of 13
    No
    Yes
    Saccharomyces cerevisiae (Brewer's yeast) (1)
    Scer\ATP6
    11 of 13
    Yes
    Yes
    Schizosaccharomyces pombe (Fission yeast) (1)
    Spom\ScpofMp06
    9 of 12
    Yes
    Yes
    Escherichia coli (enterobacterium) (1)
    Ecol\atpB
    2 of 11
    Yes
    Yes
    Other Organism Orthologs (via OrthoDB)
    Data provided directly from OrthoDB:mt:ATPase6. Refer to their site for version information.
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v9.1)
    Human Disease Associations
    FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    mt:ATPase61
    model of  mitochondrial metabolism disease
    is exacerbated by SurG7604
    (Fogle et al., 2016)
    is exacerbated by SurMB07175
    (Fogle et al., 2016)
    model of  mitochondrial encephalomyopathy
    is exacerbated by IdhRNAi.UAS.cUa
    (Fogle et al., 2019)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MT-ATP6; mitochondrially encoded ATP synthase membrane subunit 6
    12 of 14
    516060
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Interactions Browser
        Summary of Genetic Interactions
        esyN Network Diagram
        Show/hide secondary interactors 
        (data from AllianceMine provided by esyN)
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        mt:ATPase6
        enhanceable
        sesB
        (Celotto et al., 2006)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        sesB
        enhanceable
        mt:ATPase6
        (Celotto et al., 2006)
        External Data
        Subunit Structure (UniProtKB)
        F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.
        (UniProt, P00850 )
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        mitochondrion_genome
        Recombination map
        Cytogenetic map
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (4)
        Genomic Clones (0)
         
          cDNA Clones (0)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                  Other clones
                    RNAi and Array Information
                    Linkouts
                    DRSC - Results frm RNAi screens
                    Antibody Information
                    Laboratory Generated Antibodies
                     
                    Commercially Available Antibodies
                     
                    Cell Line Information
                    Publicly Available Cell Lines
                     
                      Other Stable Cell Lines
                       
                        Other Comments

                        New annotation (CG34073) in release 4.3 of the genome annotation.

                        (FlyBase Genome Annotators, 2005)

                        Amino acid substitutions found specifically in mt:ATPase6 are at sites not conserved among mammals, yeast or E.coli. These substitutions can be explained by neutral mutations and random genetic drift.

                        (Kaneko et al., 1993)
                        Relationship to Other Genes
                        Source for database merge of
                        Additional comments
                        Nomenclature History
                        Source for database identify of
                        Nomenclature comments
                        Etymology
                        Synonyms and Secondary IDs (14)
                        Reported As
                        Symbol Synonym
                        A6
                        (Chen et al., 2012)
                        ATP6
                        (Camus et al., 2023, Dowling and Wolff, 2023, Anderson et al., 2022, Salminen and Vale, 2020, Fogle et al., 2019, Markantone et al., 2018, Matsushima et al., 2017, Bratic et al., 2016, Fogle et al., 2016, Van Bortle et al., 2015, Baggio et al., 2014, Towheed et al., 2014, Liu et al., 2012, Aw et al., 2011, Bratic et al., 2011, Celotto et al., 2011, Palladino et al., 2007, Celotto et al., 2006, Frank et al., 2006, Ballard, 2000)
                        ATPase6
                        (Gnainsky et al., 2021, Vaught et al., 2020, Tsai et al., 2016, Jaiswal et al., 2015, Montooth et al., 2009, Celotto et al., 2006, Roberti et al., 2006, Weinreich and Rand, 2000, Nachman, 1998, Calleja et al., 1993)
                        CG34073
                        (Çiçek et al., 2016, Foriel et al., 2015, Ni et al., 2010.12.1, Perkins et al., 2009, FlyBase Genome Annotators, 2005)
                        atp6
                        (Aradska et al., 2015, Stewart and Beckenbach, 2009, Torres et al., 2009)
                        atp6ase
                        (Mossman et al., 2016)
                        mt:ATP6
                        (Klucnika et al., 2023, Clemente et al., 2022)
                        mt:ATPase6
                        (Sriskanthadevan-Pirahas et al., 2023, Huang et al., 2022, Murari et al., 2022, Higareda Alvear et al., 2021, Xie et al., 2021, Hope et al., 2020, Xu et al., 2020, Barry and Thummel, 2016, Fear et al., 2016, Mossman et al., 2016, Barnes et al., 2010)
                        mtATP6
                        (Gehrke et al., 2015, Korey, 2007, Kotchey and Palladino, 2007)
                        Name Synonyms
                        ATPase 6
                        (Loewen and Ganetzky, 2018, Ballard, 2000)
                        ATPase 6 gene
                        (Rand and Kann, 1996)
                        Mitochondrial ATPase subunit 6
                        (Mossman et al., 2016)
                        mitochondrial ATP6
                        (Celotto et al., 2006)
                        mitochondrial ATPase subunit 6
                        Secondary FlyBase IDs
                        • FBgn0002867
                        Datasets (0)
                        Study focus (0)
                        Experimental Role
                        Project
                        Project Type
                        Title
                        Study result (0)
                        Result
                        Result Type
                        Title
                        External Crossreferences and Linkouts ( 36 )
                        Sequence Crossreferences
                        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                        Other crossreferences
                        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                        FlyMine - An integrated database for Drosophila genomics
                        InterPro - A database of protein families, domains and functional sites
                        KEGG Genes - Molecular building blocks of life in the genomic space.
                        MARRVEL_MODEL - MARRVEL (model organism gene)
                        Linkouts
                        DroID - A comprehensive database of gene and protein interactions.
                        DRSC - Results frm RNAi screens
                        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
                        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                        Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
                        References (78)