FlyBase Gene Report: Dmel\Acbp2

General Information

Symbol

Dmel\Acbp2

Species

D. melanogaster

Name

Acyl-CoA binding protein 2

Annotation Symbol

CG8627

Feature Type

protein_coding_gene

FlyBase ID

FBgn0010387

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

Binds medium- and long-chain acyl-CoA esters with very high affinity and may function as an intracellular carrier of acyl-CoA esters (By similarity). May be involved in energy metabolism in a manner that depends on the substrate used for energy production. Dbi and its metabolites are involved in the regulation of multiple biological processes. (UniProt, P42281)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Dbi, ACBP, Diazepam-binding inhibitor

Key Links

Genomic Location

Cytogenetic map

65E4-65E4

Sequence location

3L:7,131,863..7,133,463 [+]

Recombination map

3-19

RefSeq locus

NT_037436 REGION:7131863..7133463

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables fatty-acyl-CoA binding

inferred from electronic annotation with InterPro:IPR000582, InterPro:IPR035984

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity

(Kolmer et al., 1994)

enables long-chain fatty acyl-CoA binding

inferred from biological aspect of ancestor with PANTHER:PTN004628734

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in fatty acid metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000592996

(GO Reference Genome Project, 2011-)

involved_in intracellular acyl-CoA homeostasis

inferred from sequence or structural similarity

(Kolmer et al., 1994)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

ACYL-COA BINDING PROTEINS

Protein Family (UniProt)

Belongs to the ACBP family. (P42281)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ACYL-COA BINDING PROTEINS -: The Acyl-CoA-binding proteins constitute a family of conserved proteins that bind long-chain acyl-Coenzyme A esters with high affinity. They are involved in multiple cellular tasks including intracellular acyl-CoA transport, modulation of fatty acid biosynthesis, vesicular trafficking and gene regulation. (Adapted from PMID:16018771.)

Protein Function (UniProtKB)

(UniProt, P42281)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Acbp2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P42281)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0076915

420

FBtr0076916

386

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Acbp2-PA

FBpp0076624

9.6

8.90

FBpp0076625

9.6

8.90

P42281

NP_523952

AAN12074

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

86 aa isoforms: Acbp2-PA, Acbp2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P42281

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acbp2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dbi_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.12

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

amnioserosa

(Fisher et al., 2012)

embryonic stage 11 -- 12

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic midgut chamber

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Acbp2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Acbp2

View exonic expression by tissue for Dmel\Acbp2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010387

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38784/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010387

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010387

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010387

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8627

Flygut - An atlas of the Drosophila adult midgut

FBgn0010387

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Acbp2

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Acbp2

Transgenic constructs containing regulatory region of Acbp2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel6110

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^Mef2.PR

Acbp2^GD13725

viable, with Scer\GAL4^pnr-MD237

Acbp2^GD13725

viable, with Scer\GAL4^tin.CΔ4

Acbp2^GD13725

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (10)

Hsap\DBI

10 of 14

Yes

8 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

Yes

Hsap\ESPNL

1 of 14

Hsap\MTPN

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Dbi

10 of 14

Yes

Rnor\Acbd7

9 of 14

Rnor\Dbil5

6 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Rnor\Eci3

1 of 14

Rnor\Espnl

1 of 14

Rnor\Mtpn

1 of 14

Mus musculus (laboratory mouse) (10)

Mmus\Dbi

9 of 14

Yes

Mmus\Acbd7

8 of 14

Mmus\Dbil5

6 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Mmus\Mtpn

1 of 14

Xenopus tropicalis (Western clawed frog) (6)

Xtro\dbi

5 of 13

Yes

4 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100498463

1 of 13

Yes

Danio rerio (Zebrafish) (8)

Drer\dbi

10 of 14

Yes

8 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\acbp-1

13 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP007460

11 of 12

Yes

6 of 12

2 of 12

2 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (7)

Atha\ACBP6

11 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ACB1

12 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC1539.06

12 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Acbp2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

11 of 13

8 of 13

6 of 13

4 of 13

4 of 13

4 of 13

2 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DBI; diazepam binding inhibitor, acyl-CoA binding protein

10 of 14

125950

ACBD7; acyl-CoA binding domain containing 7

8 of 14

ACBD4; acyl-CoA binding domain containing 4

3 of 14

619968

ACBD5; acyl-CoA binding domain containing 5

3 of 14

616618

RETINAL DYSTROPHY WITH LEUKODYSTROPHY; RDLKD

retinal dystrophy with leukodystrophy

ECI2; enoyl-CoA delta isomerase 2

3 of 14

608024

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0010387

MIST (protein-protein) - An integrated Molecular Interaction Database

38784

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-19

Cytogenetic map

65E4-65E4

Sequence location

3L:7,131,863..7,133,463 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65E4-65E4

Limits computationally determined from genome sequence between P{lacW}l(3)L4060^L4060 and P{PZ}corn⁰⁴²⁰²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

65E-65E

(Roos, 1993.12.30)

65E3-65E9

(determined by in situ hybridisation)

(Kolmer et al., 1994)

Experimentally Determined Recombination Data

Location

3-19

(FlyBase, 2016)

3-16.6

(Comeron et al., 2012)

(Roos, 1993.12.30)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

64850

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05723}attP40

VDRC

v23679

w¹¹¹⁸; P{GD13725}v23679

v23680

w¹¹¹⁸; P{GD13725}v23680

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (17)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH39533

Other clones

DPiM_RH39533

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0010387

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0010387

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Isolated from a genomic library using a rat DBI cDNA as a probe, under low stringency conditions.

(Kolmer et al., 1994)

Dbi has been cloned and sequenced, and its expression pattern has been analysed.

(Kolmer et al., 1994)

Relationship to Other Genes

Source for database merge of

Source for merge of: Dbi BcDNA:RH39533

(FlyBase, 1996-)

Source for merge of: Dbi anon-WO0172774.131

(FlyBase, 1996-)

Additional comments

Source for merge of Dbi BcDNA:RH39533 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Source for merge of Dbi anon-WO0172774.131 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Acbp2 Dbi

(Burton et al., 2005)

Nomenclature comments

FlyBase curator comment: Renamed from 'Dbi' because: (i) there's no evidence that the fly gene product is a "Diazepam-binding inhibitor"; (ii) one of the original authors that characterized the D. melanogaster gene (see FBrf0076972) later said that "the binding of Acyl-CoA is the only function of DBI/ACBP which has been supported with remarkable body of experimental evidence...it is highly unlikely that the real function of ACBP/DBI would be binding of diazepam" (FBrf0129015); and (iii) it makes sense to name this gene using the "Acbp" prefix as part of the "Acyl-CoA binding protein 1" family identified in the this paper.

(Burton et al., 2005)

Etymology

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

ACBP

(Toprak et al., 2020, Wadum et al., 2002, Kolmer, 2000.5.1)

Acbp2

(Li and Karpac, 2023, Cho et al., 2020)

BcDNA:RH39533

CG8627

(Zhang et al., 2021, Cammarato et al., 2011, Zheng et al., 2011, Perkins et al., 2009, Kolmer, 2000.5.1)

DBI

(Kolmer, 2000.5.1, Kolmer et al., 1994, Roos et al., 1994, Roos, 1993.12.30, Roos, 1993.12.30)

Dbi

(Li et al., 2024, Blatt et al., 2021, Pang et al., 2021, Zhang et al., 2021, Yuan et al., 2015, McPhee et al., 2013, Ladoukakis et al., 2011, Zheng et al., 2011, Seugnet et al., 2009)

DmDBI

(Majerowicz et al., 2016)

anon-WO0172774.131

dbi

(Nazario-Toole et al., 2018, Kurmangaliyev et al., 2015, Ghezzi et al., 2013)

Name Synonyms

Acyl-CoA binding protein 2

Diazepam-binding inhibitor

(Cammarato et al., 2011)

acyl-CoA binding protein

(Kolmer, 2000.5.1)

acyl-CoA-binding protein

(Toprak et al., 2020)

diazepam binding inhibitor

(Hetru, 1993.11.3)

diazepam-binding protein

(Kolmer, 2000.5.1)

endozepine

(Kolmer, 2000.5.1)

Secondary FlyBase IDs

FBgn0046463
FBgn0047016

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38784

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P42281

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P42281

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P42281

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0010387

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38784/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0010387

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0010387

FlyMine - An integrated database for Drosophila genomics

FBgn0010387

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8627

MARRVEL_MODEL - MARRVEL (model organism gene)

38784

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0010387

DroID - A comprehensive database of gene and protein interactions.

FBgn0010387

DRSC - Results frm RNAi screens

FBgn0010387

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dbi_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010387

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0010387

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8627

Flygut - An atlas of the Drosophila adult midgut

FBgn0010387

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012626

MIST (protein-protein) - An integrated Molecular Interaction Database

38784

References (56)

The following external sites may use different assemblies or annotations than FlyBase.

-0.12

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