Sulf1KO;Cherry homozygous (and to a lesser degree but still significant also heterozygous) mutants as well as Sulf1KO;Cherry/Sulf1ΔP1 transheterozygotes display increased number of dividing intestinal stem cells (ISCs) in posterior midgut during normal homeostasis, the number of ISCs in the midgut of Sulf1KO;Cherry homozygotes is, however, comparable to controls.
Sulf1KO;Cherry mutants also show prolonged activation of ISC division during regeneration induced by Pseudomonas entomophila infection.
Sulf1KO.Cherry/Sulf1ΔP1 has increased occurrence of cell division | adult stage phenotype, suppressible by Scer\GAL4NP6267/Stat92ERNAi.UAS
Sulf1KO.Cherry/Sulf1ΔP1 has increased occurrence of cell division | adult stage phenotype, suppressible by Scer\GAL4NP6267/EgfrDN.UAS.cBa
Sulf1KO.Cherry/Sulf1ΔP1 has increased occurrence of cell division | adult stage phenotype, suppressible by Scer\GAL4NP6267/ptcUAS.cJa
Sulf1KO.Cherry/Sulf1ΔP1 has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4NP6267/Stat92ERNAi.UAS
Sulf1KO.Cherry/Sulf1ΔP1 has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4NP6267/EgfrDN.UAS.cBa
Sulf1KO.Cherry/Sulf1ΔP1 has adult posterior midgut epithelium phenotype, suppressible by Scer\GAL4NP6267/ptcUAS.cJa
The increased division of intestinal stem cells in the adult posterior midgut during normal homeostasis characteristic for Sulf1KO;Cherry/Sulf1ΔP1 mutants can be suppressed by expression of any of the following: Stat92EdsRNA.Scer\UAS, EgfrDN.Scer\UAS.cBa or ptcScer\UAS.cJa under the control of Scer\GAL4NP6267 (using tub-Gal80[ts] to limit the time of expression to adulthood).