FlyBase Allele Report: Dmel\Vps35[UAS.Tag:HA]

General Information

Symbol

Dmel\Vps35^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0296524

Feature type

allele

Associated gene

Dmel\Vps35

Associated Insertion(s)

Carried in Construct

PBac{UAS-Vps35.HA}

Also Known As

UAS-Vps35

Key Links

Transgenic product class

wild_type

(Wang et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wang et al., 2014)

Mutagen

in vitro construct

(Wang et al., 2014)

Progenitor genotype

Carried in construct

PBac{UAS-Vps35.HA}

Cytology

Description

UASt regulatory sequences drive expression of full-length Vps35 coding sequences tagged at the C-terminal with Tag:HA.

(Wang et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Vps35

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Leigh disease

modeled by Lrpprc2^A

(Jaiswal et al., 2015)

ameliorates Parkinson's disease 14

modeled by iPLA2-VIA^Δ174

(Lin et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult flies expressing Vps35^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ninaE.PU show no significant difference in the number of rhodopsin1-containing vesicles per ommatidium during illumination compared to controls.

(Thakur et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Vps35^UAS.Tag:HA/Scer\GAL4^nSyb.PS is a suppressor | partially of abnormal neurophysiology | adult stage phenotype of Vps29¹

(Ye et al., 2020)

Vps35^UAS.Tag:HA/Scer\GAL4^Act5C.PI is a suppressor | partially of bang sensitive phenotype of iPLA2-VIA^Δ174

(Lin et al., 2018)

Vps35^UAS.Tag:HA/Scer\GAL4^Act5C.PI is a suppressor | partially of abnormal neurophysiology | progressive phenotype of iPLA2-VIA^Δ174

(Lin et al., 2018)

Scer\GAL4^ninaE.PU/Vps35^UAS.Tag:HA is a suppressor | partially of abnormal neurophysiology | somatic clone | adult stage | conditional phenotype of Lrpprc2^A

(Jaiswal et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Vps35^UAS.Tag:HA/Scer\GAL4^nSyb.PS is a suppressor | partially of retina | adult stage phenotype of Vps29¹

(Ye et al., 2020)

Vps35^UAS.Tag:HA/Scer\GAL4^Act5C.PI is a suppressor | partially of retina | progressive phenotype of iPLA2-VIA^Δ174

(Lin et al., 2018)

Scer\GAL4^ninaE.PU/Vps35^UAS.Tag:HA is a suppressor of retina | adult stage | progressive | conditional phenotype of Pld^3.1

(Thakur et al., 2016)

Scer\GAL4^ninaE.PU/Vps35^UAS.Tag:HA is a suppressor of ommatidium | adult stage | progressive | conditional phenotype of Pld^3.1

(Thakur et al., 2016)

Scer\GAL4^Act5C.PU, w^JF01901, Vps35^UAS.Tag:HA is a suppressor of rhabdomere phenotype of cm¹

(Wang et al., 2014)

Scer\GAL4^Act5C.PU, w^JF01901, Vps35^UAS.Tag:HA is a suppressor of ommatidium phenotype of cm¹

(Wang et al., 2014)

Scer\GAL4^Act5C.PU, w^JF01901, Vps35^UAS.Tag:HA is a suppressor of rhabdomere phenotype of norpA^P24

(Wang et al., 2014)

Scer\GAL4^Act5C.PU, w^JF01901, Vps35^UAS.Tag:HA is a suppressor of ommatidium phenotype of norpA^P24

(Wang et al., 2014)

Scer\GAL4^Act5C.PU, w^JF01901, Vps35^UAS.Tag:HA is a suppressor of photoreceptor neuron phenotype of norpA^P24

(Wang et al., 2014)

Scer\GAL4^Act5C.PU, w^JF01901, Vps35^UAS.Tag:HA is a suppressor of photoreceptor neuron phenotype of cm¹

(Wang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The light-dependent retinal degeneration observed in Pld^3.1 homozygous adults is suppressed by expression of Vps35^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ninaE.PU in the mutant background.

(Thakur et al., 2016)

Expression of Vps35^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^ninaE.PU significantly partially suppresses decreases in ERG amplitude seen in 7 day old Lrpprc2^A/Lrpprc2^A flies raised under constant light conditions.

(Jaiswal et al., 2015)

Overexpression of Vps35^{Scer\UAS.T:Ivir\HA1} in a Scer\GAL4^Act5C.PU w^JF01901 background, significantly restores rhabdomere number in norpA³⁶ mutants that have been continuously exposed to white light for 6 days.

Overexpression of Vps35^{Scer\UAS.T:Ivir\HA1} in a Scer\GAL4^Act5C.PU w^JF01901 background, significantly restores rhabdomere number and suppresses the ERG amplitude defects found in cm¹ mutants that have been continuously exposed to white light for 6 days.

(Wang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67152

y¹ w^*; PBac{UAS-Vps35.HA}VK00033

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Vps35^{Scer\UAS.T:Ivir\HA1}

Vps35^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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