UAS regulatory sequences drive expression of a DsRed(Unk) fluorescent protein. A genomic fragment containing the mir-190 pre-miRNA hairpin has been inserted into the DsRed(Unk) 3'UTR.
Expression of mir-190Scer\UAS.T:Disc\RFP under the control of Scer\GAL4Bx-MS1096 results in folded wings with blisters.
Expression of mir-190Scer\UAS.T:Disc\RFP under the control of Scer\GAL4sd-SG29.1 results in wing notching or an incomplete margin. Wings are often blistered and vein L5 is thicker than normal.
Expression of mir-190Scer\UAS.T:Disc\RFP under the control of Scer\GAL4ptc-559.1 results in veins L3 and L4 being fused due to lack of intervein tissue. Some lethality is seen.
Expression of mir-190Scer\UAS.T:Disc\RFP under the control of Scer\GAL4ptc-559.1 results in undergrowth of the L3-L4 intervein region.
Expression of mir-190Scer\UAS.T:Disc\RFP under the control of Scer\GAL4Bx-MS1096 results in wing vein thickening.
Expression of mir-190Scer\UAS.T:Disc\RFP under the control of Scer\GAL4sd-SG29.1 results in wing notching.