FlyBase Allele Report: Dmel\hep[NIG.4353R]

General Information

Symbol

Dmel\hep^NIG.4353R

Species

D. melanogaster

Name

FlyBase ID

FBal0269068

Feature type

allele

Associated gene

Dmel\hep

Associated Insertion(s)

Carried in Construct

P{NIG.4353R}

Also Known As

UAS-hep-IR, UAS-hep RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Sekine et al., 2011, Ishimaru et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Sekine et al., 2011, Ishimaru et al., 2004)

Mutagen

in vitro construct

(Sekine et al., 2011, Ishimaru et al., 2004, Ishimaru et al., 2004)

Progenitor genotype

Carried in construct

P{NIG.4353R}

Cytology

Description

UASt drives expression of a hep RNAi construct.

(Sekine et al., 2011)

UASt sequences drive expression of an inverted repeat of hep coding sequences (from GenBank:U05240 nucleotides 646 to 1346).

(Ishimaru et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-4353R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by Ras85D^V12.UAS, l(2)gl⁴

(Igaki et al., 2006)

ameliorates human immunodeficiency virus infectious disease

modeled by HIV-1\Vpu^UAS.cLa

(Marchal et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval hemolymph | third instar larval stage, with Scer\GAL4^Act5C.PU

(Bidla et al., 2007)

thorax, with Scer\GAL4^pnr-MD237

(Ishimaru et al., 2004)

Detailed Description

Statement

Reference

Hemolymph clots from third instar larvae expressing hep^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PU show a complete lack of melanization.

(Bidla et al., 2007)

When hep^{dsRNA.Scer\UAS} is driven by Scer\GAL4^pnr-MD237 mutant flies have a split thorax.

(Ishimaru et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

hep^NIG.4353R/Scer\GAL4^ptc.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^ptc.PU, fmt^GD6928

(Ma et al., 2017)

hep^NIG.4353R, Scer\GAL4^dpp.blk1 is a suppressor | partially of visible phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

hep^NIG.4353R, Scer\GAL4^pnr-MD237 is a suppressor of abnormal body color phenotype of Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

hep^NIG.4353R, Scer\GAL4^dpp.blk1 is a suppressor of visible phenotype of Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+]

(Neisch et al., 2010)

hep^NIG.4353R, Scer\GAL4^dpp.blk1 is a suppressor of increased cell death phenotype of Rho1^UAS.cMa, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of BacA\p35^GMR.PH, Ras85D^KP

(Wu et al., 2009)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of BacA\p35^GMR.PH, Ras85D^KP

(Wu et al., 2009)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Ras85D^KP

(Wu et al., 2009)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of hid⁰⁵⁰¹⁴/Df(3L)H99, Ras85D^KP

(Wu et al., 2009)

hep^NIG.4353R, Scer\GAL4^Act5C.PI is a suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, l(2)gl⁴

(Igaki et al., 2006)

NOT Suppressor of

Statement

Reference

hep^NIG.4353R/Scer\GAL4^ptc.PU is a non-suppressor of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^ptc.PU, lic^UAS.ORF

(Sun et al., 2019)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

hep^NIG.4353R/Scer\GAL4^ptc.PU is a suppressor | partially of anterior crossvein phenotype of Scer\GAL4^ptc.PU, fmt^GD6928

(Ma et al., 2017)

hep^NIG.4353R, Scer\GAL4^dpp.blk1 is a suppressor | partially of wing phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

hep^NIG.4353R, Scer\GAL4^pnr-MD237 is a suppressor of adult thorax phenotype of Ask1^{ΔN.UAS.Tag:FLAG}, Scer\GAL4^pnr-MD237

(Sekine et al., 2011)

hep^NIG.4353R, Scer\GAL4^dpp.blk1 is a suppressor of wing blade phenotype of Moe^{RNAi.327-775.UAS}, Scer\GAL4^dpp.blk1, puc^E69/puc[+]

(Neisch et al., 2010)

hep^NIG.4353R, Scer\GAL4^dpp.blk1 is a suppressor of wing disc | third instar larval stage phenotype of Rho1^UAS.cMa, Scer\GAL4^dpp.blk1

(Neisch et al., 2010)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Ras85D^KP

(Wu et al., 2009)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of hid⁰⁵⁰¹⁴/Df(3L)H99, Ras85D^KP

(Wu et al., 2009)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of BacA\p35^GMR.PH, Ras85D^KP

(Wu et al., 2009)

NOT Suppressor of

Statement

Reference

hep^NIG.4353R/Scer\GAL4^ptc.PU is a non-suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc.PU, lic^UAS.ORF

(Sun et al., 2019)

hep^NIG.4353R/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The partial or complete loss of the wing anterior crossvein in flies expressing fmt^GD6928 under the control of Scer\GAL4^ptc.PU is partially suppressed by co-expression of hep^NIG.4353R.

(Ma et al., 2017)

Co-expression of hep^NIG.4353R does not suppress the small, rough eye phenotype found upon expression of nopo^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

(Ma et al., 2012)

Co-expression of hep^NIG.4153R does not suppress the thorax pigmentation defects seen in Pk92B^{ΔN.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4^pnr-MD237 flies.

(Sekine et al., 2011)

Co-expression of hep^{dsRNA.Scer\UAS} suppresses the ectopic apoptosis in cells expressing Rho1^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1.

(Neisch et al., 2010)

The eye phenotype in Ras85D^KP mutant flies is partially suppressed by ectopic expression of hep^{dsRNA.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF.

Expression of hep^{dsRNA.Scer\UAS} under the control of Scer\GAL4 in W⁰⁵⁰¹⁴/Df(3L)H99 mutant flies does not result in an eye phenotype.

Expression of hep^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^GMR.PF) in flies carrying Df(3L)H99/W⁰⁵⁰¹⁴ in a Ras85D^KP mutant background strongly suppresses the eye phenotype.

(Wu et al., 2009)

Expression of hep^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^Act5C.PI) using the FLP/FRT system strongly suppresses metastatic behaviour in l(2)gl⁴ mutants expressing Ras85D^V12.Scer\UAS.

(Igaki et al., 2006)

Xenogenetic Interactions

Statement

Reference

Co-expression of hep^NIG.4353R partially suppresses the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

(Marchal et al., 2012)

Expression of hep^{dsRNA.Scer\UAS} under the control of Scer\GAL4 in flies expressing BacA\p35^GMR.PH does not result in an eye phenotype.

Expression of hep^{dsRNA.Scer\UAS} (under the control of Scer\GAL4^GMR.PF) and BacA\p35^GMR.PH in a Ras85D^KP mutant background strongly suppresses the eye phenotype, and much reduced levels of cell death are observed.

(Wu et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

hep^NIG.4153R

hep^NIG.4353R

hep^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

FBal0175651
FBal0263926

References (21)

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