FB2024_04 , released June 25, 2024
Allele: Dmel\fweUASp.Lose-A.C.Tag:HA
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General Information
Symbol
Dmel\fweUASp.Lose-A.C.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0247689
Feature type
allele
Associated gene
Dmel\fwe
Associated Insertion(s)
Carried in Construct
P{UASp-fwe.Lose-A.HA-C}
Key Links
Transgenic product class
wild_type
(Rhiner et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Rhiner et al., 2010)
Mutagen
in vitro construct
(Rhiner et al., 2010)
Progenitor genotype
Carried in construct
P{UASp-fwe.Lose-A.HA-C}
Cytology
Description

UASp regulatory sequences drive expression of the "Lose-A" isoform of fwe tagged with Tag:HA at its C-terminus.

(Rhiner et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
fwe
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The amount of cell delamination seen in the 'loser' cells when cell competition is induced in the pupal notum through generation of clones expressing fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1 under the control of Scer\GAL4Scer\FRT.Act5C in wild type tissue correlates with the amount of surface the fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1-expressing loser cell shares with the wild type winners. The amount of shared surface does not affect delamination rates in wild type controls. The compactness of fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1 clones is unchanged compared with wild type clones in wild type tissue.

      There is no increase in cell elimination at the anterior-posterior border compared with controls when fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1 is expressed under the control of Scer\GAL4dpp.PU in the anterior compartment of the wing disc, due to high tension at the anterior-posterior boundary.

      (Levayer et al., 2015)

      Expression of fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1 under the control of Scer\GAL4Act.PU in clones of cells in the wing imaginal disc induces apoptosis and reduces clone survival.

      (Petrova et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A zip2/zipEbr mutant background (which reducing tension in cells) induces cell death at the anterior boundary when fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1 is expressed under the control of Scer\GAL4dpp.PU in the anterior compartment of the wing disc.

      (Levayer et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      fweScer\UAS.P\T.Lose-A.C.T:Ivir\HA1
      fweScer\UAS.T:Ivir\HA.cRb
      fweScer\UAS.cRb.T:Ivir\HA1
      fweUASp.Lose-A.C.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (3)