FB2024_03 , released June 25, 2024
Allele: Dmel\ykiUAS.EGFP,Tag:HA
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General Information
Symbol
Dmel\ykiUAS.EGFP,Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0239730
Feature type
allele
Associated gene
Dmel\yki
Associated Insertion(s)
Carried in Construct
P{UAS-yki.GFP}
Also Known As
UAS-yki-GFP, UAS-yki, UAS-yki.GFP, UAS-yki:GFP, UAS-Yki::GFP
Key Links
Transgenic product class
wild_type
(Oh and Irvine, 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Oh and Irvine, 2008)
Mutagen
in vitro construct
(Oh and Irvine, 2008)
Progenitor genotype
Carried in construct
P{UAS-yki.GFP}
Cytology
Description

UASt regulatory sequences drive expression of yki which is tagged with EGFP and is tagged at the C-terminal end with Tag:HA.

(Oh and Irvine, 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
yki
Tagged with
EGFP
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing ykiUAS.EGFP,Tag:HA under the control of Scer\GAL4GMR.PFa induces a mild rough eye phenotype; in agreement, the eye disc shows increased numbers of mitotic cells in the GMR-positive region, as compared to controls.

      (Yeom et al., 2020)

      Expressing ykiUAS.EGFP,Tag:HA under the control of Scer\GAL4ptc.PU leads to a significant increase in size of the wing blade domain between L3 and L4 veins.

      (Kim and Choi, 2019)

      Expression of wild-type ykiScer\UAS.cHa accelerates oocyte border cell migration.

      (Lucas et al., 2013)

      Expression of ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4GMR.PF does not result in any obvious eye overgrowth.

      Clones in the wing disc expressing ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4Act5C.PI show irregular shapes and are normal in size.

      (Oh and Irvine, 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones expressing ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4tub.PU do not have suppressed growth.

      (Suijkerbuijk et al., 2016)

      The reduction in total cell number per clone and reduction in number of Dl-positive intestinal stem cells per clone seen in MARCM clones in the intestine of lin-28Δ1 adults is not rescued by expression of ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4tub.PU.

      (Chen et al., 2015)

      Co-expression of hipkScer\UAS.T:Ivir\HA1 and ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4hh-Gal4 enhances the wing disc overgrowth seen when either construct is expressed alone.

      (Chen and Verheyen, 2012)

      Clones co-expressing hpoScer\UAS.T:Zzzz\FLAG and ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4Act5C.PI in the wing disc are tiny and rarely detected.

      (Oh and Irvine, 2008)
      Xenogenetic Interactions
      Statement
      Reference

      The additional co-expression of ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6Scer\UAS.T:Hsap\MYC and Hsap\UBE3AScer\UAS.cRa under the control of Scer\GAL4GMR.PU.

      (Padash Barmchi et al., 2016)
      Complementation and Rescue Data
      Rescues

      ykiUAS.EGFP,Tag:HA/Scer\GAL4arm.PS rescues ykiB5

      (Oh and Irvine, 2008)

      Scer\GAL4ci.PC/ykiUAS.EGFP,Tag:HA rescues ykiB5

      (Oh and Irvine, 2008)
      Comments

      Expression of ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4arm.PS rescues the lethality of ykiB5.

      Expression of ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 under the control of Scer\GAL4ci.PC rescues the growth and viability of ykiB5 clones in the wing disc.

      (Oh and Irvine, 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ykiScer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1
      ykiUAS.EGFP,Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (18)