UASt regulatory sequences drive expression of an inverted repeat.
Expression of pumKK109048 under the control of Scer\GAL4ChAT.7.4 induces a seizure phenotype in third instar larvae subjected to an electroshock - the larvae become temporarily paralyzed with recovery time significantly longer compared to controls. Scer\GAL4eve.RRa-driven expression leads to a significant increase of the persistent voltage-gated Na[+] current (I[[NaP]]) in aCC neurons, whereas the transient voltage-gated Na[+] current (I[[NaT]]) is not changed compared to wild-type controls and neither is the persistent-to-transient ratio.
pumKK109048/Scer\GAL4ChAT.7.4 is an enhancer of paralytic | adult stage phenotype of parabss1
pumKK109048/Scer\GAL4ChAT.7.4 is an enhancer of paralytic | third instar larval stage phenotype of parabss1
pumKK109048/Scer\GAL4elav.PLu is an enhancer of paralytic | third instar larval stage phenotype of parabss1
Scer\GAL4eve.RRa/pumKK109048 is a non-enhancer of abnormal neurophysiology | third instar larval stage phenotype of parabss1
Scer\GAL4eve.RRa/pumKK109048 is a non-enhancer of larval DA1 motor neuron | third instar larval stage phenotype of parabss1
Expression of pumKK109048 driven by Scer\GAL4ChAT.7.4 worsens the paralytic phenotype of parabss1 mutants induced by either brief vortexing (in adults) or an electroshock (in third instar larvae) and further delays recovery. Similarly, expression of pumKK109048 driven by Scer\GAL4elav.PLu significantly increases the seizure duration in parabss1 larvae.
Expression of pumKK109048 driven by Scer\GAL4eve.RRa in parabss1 mutant third instar larvae has no effect on I[[NaP]] and although I[[NaT]] is mildly increased, the P:T ratio is unchanged and no difference in the severity of the seizure phenotype is observed compared to parabss1 larvae.