FlyBase Allele Report: Dmel\Nek2[KK100496]

General Information

Symbol

Dmel\Nek2^KK100496

Species

D. melanogaster

Name

FlyBase ID

FBal0230786

Feature type

allele

Associated gene

Dmel\Nek2

Associated Insertion(s)

Carried in Construct

P{KK100496}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK100496}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK100496

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Nek2^KK100496 under the control of Scer\GAL4^ey.PU does not lead to any obvious third instar larval eye disc defects (in morphogenetic furrow progression, retinal differentiation or mitotic index) or in adult eye morphology, as compared to controls.

(Martins et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Nek2^KK100496, Scer\GAL4^ey.PU is a suppressor of abnormal size | adult stage phenotype of Cdc16^GD12028, Scer\GAL4^ey.PU

(Martins et al., 2017)

Nek2^KK100496, Scer\GAL4^ey.PU is a suppressor of visible | adult stage phenotype of Cdc16^GD12028, Scer\GAL4^ey.PU

(Martins et al., 2017)

NOT Suppressor of

Statement

Reference

Nek2^KK100496, Scer\GAL4^ey.PU is a non-suppressor of increased occurrence of cell division | third instar larval stage phenotype of Cdc16^GD12028, Scer\GAL4^ey.PU

(Martins et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Nek2^KK100496, Scer\GAL4^ey.PU is a suppressor of eye phenotype of Cdc16^GD12028, Scer\GAL4^ey.PU

(Martins et al., 2017)

Nek2^KK100496, Scer\GAL4^ey.PU is a suppressor of eye disc | third instar larval stage phenotype of Cdc16^GD12028, Scer\GAL4^ey.PU

(Martins et al., 2017)

Nek2^KK100496, Scer\GAL4^ey.PU is a suppressor of eye disc morphogenetic furrow | third instar larval stage phenotype of Cdc16^GD12028, Scer\GAL4^ey.PU

(Martins et al., 2017)

Nek2^KK100496/Scer\GAL4^ey.PU is a suppressor of eye disc | third instar larval stage phenotype of Cdc16^GD12028, Scer\GAL4^dac-GAL4

(Martins et al., 2017)

Nek2^KK100496/Scer\GAL4^ey.PU is a suppressor of eye disc morphogenetic furrow | third instar larval stage phenotype of Cdc16^GD12028, Scer\GAL4^dac-GAL4

(Martins et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Nek2^KK100496 fully suppresses the adult eye defects (i.e. in eye size, morphology and polarity) and the third instar larval eye disc morphogenetic furrow progression and retinal differentiation impairment (but not the associated increased mitotic index) induced by the expression of Cdc16^GD12028 under the control of Scer\GAL4^ey.PU.

The co-expression of Nek2^KK100496 also suppresses the third instar larval eye disc morphogenetic furrow progression and retinal differentiation impairment induced by the expression of Cdc16^GD12028 under the control of Scer\GAL4^dac-GAL4.

(Martins et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v103408

P{KK100496}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nek2^KK100496

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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