FB2024_04 , released June 25, 2024
Allele: Dmel\tefuKK100008
Open Close
General Information
Symbol
Dmel\tefuKK100008
Species
D. melanogaster
Name
FlyBase ID
FBal0230533
Feature type
allele
Associated gene
Dmel\tefu
Associated Insertion(s)
Carried in Construct
P{KK100008}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Keleman et al., 2009.8.5)
Mutagen
in vitro construct
(Keleman et al., 2009.8.5)
Progenitor genotype
Carried in construct
P{KK100008}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-KK100008
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  ataxia telangiectasia
      CEA
      (Eidhof et al., 2023, Park et al., 2015)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of tefuKK100008 for 3 days during larval development under the combined control of Scer\GAL4hh.PU and Gal80[ts] leads to third instar larval wing discs exhibiting a decreased DNA damage response (phospho-H2Av) upon irradiation.

      (Murcia et al., 2019)

      The adulthood-only expression of tefuKK100008 under the control of Scer\GAL4esg-NP5130 (in combination with Gal80[ts], for the temporal control of expression) leads to a severe decrease in life-span, as compared to controls; it also results in larger and rounder adult midgut progenitor cells (esg-positive intestinal stem cells and enteroblasts), leads to their disappearance after three weeks of expression, and leads to a significant decrease in the frequency of intestinal stem cells undergoing DNA replication, but does not significantly affect the overall cell density in the adult midgut, the number of intestinal stem cells or the frequency of progenitor cells undergoing DNA replication, as compared to controls. Upon γ-radiation, the adult midgut progenitor cells show a small but evident increase in DNA damage, assessed by pS/TQ and γH2AvD immunofluorescence, as compared to irradiated controls.

      (Park et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      tefuKK100008
      Name Synonyms
      Secondary FlyBase IDs
        References (5)