FlyBase Allele Report: Dmel\spict[mut]

General Information

Symbol

Dmel\spict^mut

Species

D. melanogaster

Name

FlyBase ID

FBal0216953

Feature type

allele

Associated gene

Dmel\spict

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Wang et al., 2007)

Progenitor genotype

spict^EP2202

(Wang et al., 2007)

Cytology

Description

Excision of P{EP}spict^EP2202 removes the entire coding region of spict. The deletion extends 1753bp to the right of the insertion site of P{EP}spict^EP2202, deleting 44bp of the 69bp 5' UTR, the entire 1155bp of the spict coding sequence (interrupted by an intron of 498bp) and 56bp of the 236bp 3'UTR.

(Wang et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Wang et al., 2007)

abnormal neuroanatomy

(Wang et al., 2007)

decreased cell death | adult stage | male | calorie restriction conditional (with spict⁶⁵)

(Chiang et al., 2017)

fertile

(Wang et al., 2007)

viable

(Wang et al., 2007)

Phenotype Manifest In

bouton | increased number

(Wang et al., 2007)

embryonic/larval neuromuscular junction

(Wang et al., 2007)

male germline stem cell | calorie restriction conditional (with spict⁶⁵)

(Chiang et al., 2017)

posterior crossvein

(Wang et al., 2007)

spermatogonium | calorie restriction conditional (with spict⁶⁵)

(Chiang et al., 2017)

type I bouton

(Wang et al., 2007)

wing

(Wang et al., 2007)

Detailed Description

Statement

Reference

The starvation-induced death of spermatogonial cells (SGs) observed in wild-type testes is significantly reduced in spict⁶⁵/spict^mut mutants, the mutant males also fail to maintain germline stem cell (GSC) number during prolonged starvation and unlike in wild-type controls their GSC numbers steadily decline under starvation. Under fed conditions the number of GSCs in spict⁶⁵/spict^mut mutant testes is comparable to controls.

In spict⁶⁵/spict^mut mutant testes from starved flies cultured ex-vivo fewer SGs initiate cell death compared to controls and of these only 21% progress beyond the initial phase (level of vas protein decreases as LysoTracker signal begins to appear) of the SG cell death process, suggesting defects in both cell death initiation and progression.

(Chiang et al., 2017)

spict^mut homozygotes take about a day longer to reach adulthood compared to wild-type.

spict^mut mutants exhibit significant neuromuscular junction overgrowth compared to wild-type third instar larvae. At muscles 6 and 7 from segment A3, bouton numbers in spict^mut mutants are approximately double those in controls. The neuromuscular junctions in spict^mut larvae are also significantly longer and have more branches than those in controls. Neuromuscular junction overgrowth is found throughout third instar larvae and is not obviously more severe in more posterior segments. Average bouton sizes are not significantly affected in spict^mut larvae.

Fast-axonal transport is normal in spict^mut larvae.

Approximately 27% of spict^mut mutant flies exhibit interrupted posterior crossveins in both wings. Wing size is significantly smaller in this percentage of spict^mut flies compared to wild-type. The body size of crossveinless flies is not significantly reduced.

(Wang et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

spict^mut has abnormal neuroanatomy phenotype, suppressible by sax⁴/Df(2R)cn7969

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible by wit^B11/wit^A12

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible by gbb⁴/gbb¹

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible by Med⁵

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible | partially by tkv⁷/tkv[+]

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible | partially by sax⁴/sax[+]

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible | partially by gbb¹/gbb[+]

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible | partially by wit^A12/wit[+]

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible | partially by Med[+]/Med⁵

(Wang et al., 2007)

spict^mut has abnormal neuroanatomy phenotype, suppressible by tkv^k16713/tkv⁷

(Wang et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible by tkv^k16713/tkv⁷

(Wang et al., 2007)

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible by sax⁴/Df(2R)cn7969

(Wang et al., 2007)

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible by wit^B11/wit^A12

(Wang et al., 2007)

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible by gbb⁴/gbb¹

(Wang et al., 2007)

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible | partially by tkv⁷/tkv[+]

(Wang et al., 2007)

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible | partially by sax⁴/sax[+]

(Wang et al., 2007)

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible | partially by gbb¹/gbb[+]

(Wang et al., 2007)

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible | partially by wit^A12/wit[+]

(Wang et al., 2007)

spict^mut has embryonic/larval neuromuscular junction phenotype, suppressible | partially by Med[+]/Med⁵

(Wang et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

spict^mut neuromuscular junction overgrowth phenotypes are fully suppressed in tkv⁷/tkv^k16713, sax⁴/Df(2R)cn7969, wit^A12/wit^B11, gbb¹/gbb⁴ or Med⁵/Med⁵ mutants. In all cases the synaptic undergrowth phenotypes in larvae homozygous for spict^mut and the BMP pathway mutations above are indistinguishable from that of homozygous BMP pathway mutations alone. In addition, heterozygous sax⁴, tkv⁷, gbb¹, wit^A12 and Med⁵ backgrounds partially suppress the neuromuscular junction expansion of spict^mut larvae.

(Wang et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

spict^mut is rescued by spict^{UAS.mRFP(Unk)}/Scer\GAL4^elav.PLu

(Wang et al., 2007)

spict^mut is rescued by Scer\GAL4^RapGAP1-OK6/spict^{UAS.mRFP(Unk)}

(Wang et al., 2007)

spict^mut is rescued by Scer\GAL4^elav.PLu/spict^UAS.cWa

(Wang et al., 2007)

spict^mut is rescued by Scer\GAL4^RapGAP1-OK6/spict^UAS.cWa

(Wang et al., 2007)

spict^mut is rescued by Scer\GAL4^Act5C.PI/spict^UAS.cWa

(Wang et al., 2007)

Not rescued by

spict^mut is not rescued by spict^{UAS.mRFP(Unk)}/Scer\GAL4^Mhc.PW

(Wang et al., 2007)

spict^mut is not rescued by Scer\GAL4^Mhc.PW/spict^UAS.cWa

(Wang et al., 2007)

Comments

spict^mut neuromuscular junction overgrowth phenotypes are fully rescued by expression of spict^{Scer\UAS.T:Disc\RFP-mRFP} in neurons (under the control of Scer\GAL4^elav.PLu or Scer\GAL4^OK6) but not muscles (under the control of Scer\GAL4^Mhc.PW).

spict^mut neuromuscular junction overgrowth phenotypes are fully rescued by expression of spict^Scer\UAS.cWa in neurons (under the control of Scer\GAL4^elav.PLu or Scer\GAL4^OK6) but not muscles (under the control of Scer\GAL4^Mhc.PW).

Overexpression of spict^Scer\UAS.cWa under the control of Scer\GAL4^Act5C.PI completely rescues the interrupted posterior crossvein phenotype found in spict^mut flies.

(Wang et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

spict⁴¹

(Chiang et al., 2017)

spict^mut

Name Synonyms

Secondary FlyBase IDs

References (2)

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