The PBac{RB}wole04276 insertion disrupts wol translation after 80 amino acids that lack almost the entire glycosyltransferase domain of 182 residues starting at position 69.
Homozygous or wole04276/Df(2L)BSC111 larvae hatch and have a wild type cuticle by light microscopy. They eventually die after one moult without displaying any obvious phenotype.
Removal of maternal wol in wole04276 germline clones results in larvae with patterning and morphological defects, the most prominent being the failure to form a normal head skeleton. The cuticle is discontinuous and the body contours are irregular. At the edges of ruptures, the cuticle is often melanized. Specialized cuticle structures such as denticles are present.
Removal of maternal and zygotic wol in wole04276 germline clones causes additional reduction in cuticle deposition and a complete failure of denticle formation and melanization.
