FlyBase Allele Report: Dmel\Rh6[1]

General Information

Symbol

Dmel\Rh6¹

Species

D. melanogaster

Name

FlyBase ID

FBal0141562

Feature type

allele

Associated gene

Dmel\Rh6

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

natural population

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Vasiliauskas et al., 2011)

Mutagen

natural population

(FlyBase Curators, 2002-)

Progenitor genotype

Cytology

Description

Mutation in sequenced strain: deletion (-19, coding/intron).

(FlyBase Curators, 2002-)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of sensorineural hearing loss

CEA

(Senthilan et al., 2012)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates sensorineural hearing loss

modeled by Rh5²

(Senthilan et al., 2012)

model of sensorineural hearing loss

is exacerbated by Rh5²

(Senthilan et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal auditory perception | adult stage

(Senthilan et al., 2012)

abnormal behavior | larval stage

(Liu et al., 2014)

abnormal neurophysiology | adult stage

(Katana et al., 2019, Senthilan et al., 2012)

abnormal thermotaxis | third instar larval stage

(Sokabe et al., 2016)

Phenotype Manifest In

Johnston organ | third instar larval stage

(Katana et al., 2019)

Detailed Description

Statement

Reference

Rh6¹ adults exhibit antennal free fluctuations with a diminished power and an altered frequency tuning compared to controls. When stimulated with pure tones, the displacement response of mutant antennae lack the characteristic nonlinear intensity scaling compared to controls. Also the amplitude and sensitivity of compound action potentials responses are reduced in mutants compared to controls. Johnston's organ function of the mutants remain uncompromised by nutrient carotenoid depletion for six generations.

(Katana et al., 2019)

Rh5² homozygous mutant mid and late third instar (96 and 120 hr AEL, respectively) larvae show impaired temperature selection and unlike controls display no strong preference for cooler temperatures (18[o]C) in a temperature gradient. This effect does not appear to be due to developmental delay as the mutants develop with similar timing as controls, they also do not show any gross defects in the morphology of TrpA1 neurons, 18[o]C versus 28[o]C thermotaxis or crawling speed.

(Sokabe et al., 2016)

Mutant larvae show significantly decreased green-light avoidance compared to controls.

(Liu et al., 2014)

Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. The displacement response of the antenna over a range of sound particle velocities is linearised, indicating loss of mechanical amplification.

(Senthilan et al., 2012)

Photophobicity is unaffected in Rh6¹ mutants.

(Keene et al., 2011)

Rh6¹ mutant class IV dendritic arborization neurons do not exhibit any defects in light response.

(Xiang et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rh6¹, cry^b, norpA^P41 has abnormal locomotor rhythm phenotype, enhanceable by Rh5²

(Szular et al., 2012)

Rh6¹, cry^b has abnormal locomotor rhythm phenotype, enhanceable by Rh5²

(Szular et al., 2012)

NOT Enhanced by

Statement

Reference

Rh6¹ has abnormal thermotaxis | third instar larval stage phenotype, non-enhanceable by Rh5²

(Sokabe et al., 2016)

NOT suppressed by

Statement

Reference

Rh6¹ has abnormal behavior | larval stage phenotype, non-suppressible by Ggt-1^Δ1523

(Liu et al., 2014)

Enhancer of

Statement

Reference

Rh6¹ is an enhancer of abnormal locomotor rhythm phenotype of cry^b, norpA^P41

(Szular et al., 2012)

Rh5²/Rh6¹ is an enhancer of abnormal locomotor rhythm phenotype of cry^b, norpA^P41

(Szular et al., 2012)

Rh6¹ is an enhancer of abnormal locomotor rhythm phenotype of Rh5², cry^b, norpA^P41

(Szular et al., 2012)

Rh6¹ is an enhancer of abnormal locomotor rhythm phenotype of Rh5², cry^b

(Szular et al., 2012)

Rh5²/Rh6¹ is an enhancer of abnormal locomotor rhythm phenotype of cry^b

(Szular et al., 2012)

Rh6¹ is an enhancer of photoperiod response variant phenotype of ninaE¹⁷

(Hanai and Ishida, 2009)

Rh6¹ is an enhancer of photoperiod response variant phenotype of cry^b, ninaE¹⁷

(Hanai and Ishida, 2009)

NOT Enhancer of

Statement

Reference

Rh6¹ is a non-enhancer of abnormal thermotaxis | third instar larval stage phenotype of Rh5²

(Sokabe et al., 2016)

Rh6¹ is a non-enhancer of abnormal locomotor rhythm phenotype of cry^b

(Szular et al., 2012)

Rh6¹ is a non-enhancer of abnormal phototaxis phenotype of Rh5²

(Keene et al., 2011)

Rh6¹ is a non-enhancer of photoperiod response variant phenotype of cry^b

(Hanai and Ishida, 2009)

Suppressor of

Statement

Reference

Rh5², Rh5², Rh6¹, Rh6¹ is a suppressor of abnormal circadian rhythm | adult stage phenotype of cry⁰¹

(Ni et al., 2017)

NOT Suppressor of

Statement

Reference

Rh6¹ is a non-suppressor of abnormal phototaxis phenotype of Rh5²

(Keene et al., 2011)

Other

Statement

Reference

Rh5², Rh6¹ has abnormal neurophysiology | adult stage phenotype

(Senthilan et al., 2012)

Rh5², Rh6¹ has abnormal auditory perception | adult stage phenotype

(Senthilan et al., 2012)

Rh5², Rh6¹ has abnormal neuroanatomy | third instar larval stage phenotype

(Yuan et al., 2011)

Rh5², Rh6¹, sev¹ has abnormal optomotor response phenotype

(Yamaguchi et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

Rh5², Rh6¹ has dendrite | third instar larval stage phenotype

(Yuan et al., 2011)

Rh5², Rh6¹ has LNv neuron | third instar larval stage phenotype

(Yuan et al., 2011)

Rh6¹, pros¹⁷ has photoreceptor cell R7 phenotype

(Cook et al., 2003)

Rh6¹, pros¹⁷ has photoreceptor cell R8 | ectopic phenotype

(Cook et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Rh6¹, Rh5² double homozygosity rescues the circadian rhythmicity defects of cry⁰¹ homozygotes.

(Ni et al., 2017)

The impaired thermotactic behavior characteristic for Rh5² and Rh6¹ single mutant late third instar larvae (120 hr AEL) does not deteriorate further in the Rh5²;Rh6¹ double mutants.

(Sokabe et al., 2016)

The reduced green-light avoidance seen in Rh6¹ larvae is not altered if the larvae are also mutant for Ggt-1^Δ1523.

(Liu et al., 2014)

The sound threshold required to evoke a compound action potential in the antennal nerve in response in Rh5² Rh6¹ double mutants is almost twice as loud as required in single mutants. Ultrastructure of the Johnston's organ sensillum appears normal in the double mutant flies.

(Senthilan et al., 2012)

Following a 6 hour delay in their light-dark cycle, cry^b Rh6¹ flies show a similar delay in resynchronizing their evening activity peak as cry^b flies. Triple mutant cry^b Rh5² Rh6¹ flies resynchronize over a longer period than either the cry^b single or double mutants.

Following a 6 hour delay in their light-dark cycle, cry^b norpA³⁹ flies show a longer delay in resynchronizing their evening activity peak (5-7 days) compared to cry^b flies. The addition of Rh6¹ to this background extends the time required for resynchronization to beyond 7 days. The addition of both Rh5² and Rh6¹ to the cry^b norpA³⁹ background results in minimal (if any) tendency to resynchronize circadian behaviour.

Following a 6 hour advancement in their light-dark cycle, cry^b norpA³⁹ flies show a longer delay in resynchronizing their circadian behaviour (several days) compared to either single mutant. Introducing Rh6¹ to this background interferes more strongly with resynchronization, while the quadruple cry^b norpA³⁹ Rh5² Rh6¹ mutant shows the strongest phenotype.

(Szular et al., 2012)

Wild-type larvae display differences in their LN(v) dendrite length when exposed to constant darkness versus constant light. In contrast, such light induced changes are absent in Rh5², Rh6¹ double mutants.

(Yuan et al., 2011)

A Rh6¹ mutant background does not affect the ability of cry^b mutants to adapt to a new phase of green light (472-603nm) and Y-FL (475-724nm).

The retrainment of ninaE¹⁷ Rh6¹ cry^b to 6 hour delayed green light (472-603nm) and Y-FL (475-724nm) is significantly slower than that of ninaE¹⁷ cry^b mutants. Indeed some flies do not adapt to the new photoperiod within 7 days.

ninaE¹⁷ Rh5³ Rh6¹ cry^b and ninaE¹⁷ Rh5⁴ Rh6¹ cry^b quadruple mutants are not retrained by green light (472-603nm) and Y-FL (475-724nm).

(Hanai and Ishida, 2009)

sev¹, Rh5², Rh6¹ triple mutant flies do not have R7 cells and do not express function R8 opsins, therefore colour vision should be completely abolished. However, the blue/green intensity-response curve for these flies appears indistinguishable from wild-type.

(Yamaguchi et al., 2008)

R7 photoreceptors cells homozygous for pros¹⁷ and Rh6¹ undergo partial transformation towards an R8 photoreceptor fate, as judged by marker analysis, mapping of axonal projections, and proximo-distal positioning of nuclei.

(Cook et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rh6¹ is rescued by Scer\GAL4^TrpA1-AB-G4/Scer\GAL4^TrpA1-CD-G4/Rh6^UAS.cVa

(Sokabe et al., 2016)

Rh6¹/Rh6^G is rescued by Scer\GAL4^Rh6-G/Rh6^UAS.cVa

(Sokabe et al., 2016)

Rh6¹ is rescued by Rh6^+t2.6

(Senthilan et al., 2012)

Rh6¹ is rescued by Rh6^+t2.6

(Vasiliauskas et al., 2011)

Partially rescued by

Rh6¹ is partially rescued by Scer\GAL4^TrpA1-AB-G4/Rh6^UAS.cVa

(Sokabe et al., 2016)

Not rescued by

Rh6¹ is not rescued by Scer\GAL4^TrpA1-CD-G4/Rh6^UAS.cVa

(Sokabe et al., 2016)

Comments

Expression of Rh6^Scer.UAS.cVa in under the control of Scer\GAL4^Rh6-G in the Rh6¹/Rh6^G transheterozygote larvae (using the intrinsic Gal4 driver in Rh6^G) rescues the impaired thermotactic behavior characteristic for Rh6¹ mutants. The thermal preferences defects of Rh6¹ mutants are also rescued by expression of Rh6^Scer.UAS.cVa under the combined control of Scer\GAL4^TrpA1-AB-G4 and Scer\GAL4^TrpA1-CD-G4, the rescue effect is weaker when only the Scer\GAL4^TrpA1-AB-G4 driver is used and non-detectable with just the Scer\GAL4^TrpA1-CD-G4.

(Sokabe et al., 2016)

Rh6^+t2.6 rescues the auditory response defects (rescues both mechanical amplification and electrical response) seen in Rh6¹ mutants.

(Senthilan et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

2057

iso-1 : y¹; Gr22b^iso-1 Gr22d^iso-1 cn¹ CG33964^iso-1 bw¹ speck¹; MstProx^iso-1 GstD5^iso-1 Rh6¹

Kyoto

109600

Rh6¹

109601

Rh6¹ ninaE¹⁷

National Drosophila Species Stock Center

14021-0231.36

iso-1 : y¹; Gr22b^iso-1 Gr22d^iso-1 cn¹ CG33964^iso-1 bw¹ sp¹; MstProx^iso-1 GstD5^iso-1 Rh6¹

Notes on Origin

Discoverer

Present in the y¹; cn¹, bw¹, sp¹ strain, Bloomington stock number 2057, used for obtaining the whole genome sequence.

(Britt, 2002)

Comments

Mutation found during genome annotation of the strain used in the genome sequencing project.

(Misra et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Rh6¹

(Alejevski et al., 2019, Katana et al., 2019, Zanini et al., 2018, Saint-Charles et al., 2016, Liu et al., 2014, Senthilan et al., 2012, Szular et al., 2012, Keene et al., 2011, Xiang et al., 2010, Hanai and Ishida, 2009, Yamaguchi et al., 2008)

Rh6[1]

(Li et al., 2018)

rh6⁰¹

(Ni et al., 2017)

rh6¹

(Yuan et al., 2021, Leung et al., 2020, Li et al., 2020, Dombrovski et al., 2019, Qin et al., 2019, Schlichting et al., 2019, Schnaitmann et al., 2018, Sokabe et al., 2016, Vasiliauskas et al., 2011, Yuan et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (27)

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