FlyBase Allele Report: Dmel\Pcb[EP2547]

General Information

Symbol

Dmel\Pcb^EP2547

Species

D. melanogaster

Name

FlyBase ID

FBal0131769

Feature type

allele

Associated gene

Dmel\Pcb

Associated Insertion(s)

P{EP}Pcb^EP2547

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Yamazaki et al., 2014)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}Pcb^EP2547

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:9,860,670..9,860,670 [+]

(Yamazaki et al., 2014, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage | recessive

(Yamazaki et al., 2014)

partially lethal - majority die | recessive

(Yamazaki et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

PCB^EP2547/PCB^EP2547 is semi-lethal and adult flies show severe reductions in 1hr memory. PCB^EP2547/+ heterozygotes are viable and do not show significant 1hr memory defects compared to wild type (1 and 10 days old); heterozygotes show significant suppression of age-related memory impairment (AMI) seen in wild type (15 days and older).

(Yamazaki et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pcb^EP2547/PCB[+] is a suppressor of abnormal memory | adult stage phenotype of Pka-C1^UAS.Tag:FLAG, Scer\GAL4^c747

(Yamazaki et al., 2014)

NOT Suppressor of

Statement

Reference

Pcb^EP2547/PCB[+] is a non-suppressor of abnormal memory | adult stage phenotype of Scer\GAL4^nrv2.PU, Sod1^VDRC.cUa

(Yamazaki et al., 2014)

Pcb^EP2547/PCB[+] is a non-suppressor of abnormal memory | adult stage phenotype of Scer\GAL4^elav.PU, Sod1^VDRC.cUa

(Yamazaki et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

PCB^EP2547/+ suppresses 1hr memory impairment caused by overexpressing Pka-C1^{Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^c747.

Disruption of 1hr memory via glial (5 day old flies, Scer\GAL4^nrv2.PU) or neuronal (10 day old flies, Scer\GAL4^elav.PU) knockdown (Sod^VDRC.cUa) is not ameliorated in a PCB^EP2547/+ background.

(Yamazaki et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pcb^EP2547/PCB[+] is rescued by Scer\GAL4^nrv2.PU/Pcb^UAS.cYa

(Yamazaki et al., 2014)

Pcb^EP2547/PCB[+] is rescued by Scer\GAL4^repo.PU/Pcb^UAS.cYa

(Yamazaki et al., 2014)

Pcb^EP2547/PCB[+] is rescued by Scer\GAL4^{Atpα-GSG3285-1}/Pcb^UAS.cYa

(Yamazaki et al., 2014)

Not rescued by

Pcb^EP2547/PCB[+] is not rescued by Scer\GAL4^elav.PU/Pcb^UAS.cYa

(Yamazaki et al., 2014)

Comments

Age-related memory impairment (AMI) is restored by expressing PCB^Scer\UAS.cYa with glial drivers (Scer\GAL4^repo.PU or Scer\GAL4^nrv2.PU) but not a neuronal driver (Scer\GAL4^elav.PU) in PCB^EP2547/+ flies.

PCB^Scer\UAS.cYa driven by Scer\GAL4^{Atpα-GSG3285-1} in the presence of RU486 rescues PCB^EP2547/+ AMI when PCB^Scer\UAS.cYa expression is induced acutely during adult (but not larval) stages.

(Yamazaki et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

BcDNA:GH06348^EP2547

CG1516^EP2547

PCB^EP2547

Pcb^EP2547

dPC^EP2547

(Yamazaki et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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