FB2024_03 , released June 25, 2024
Allele: Dmel\apblt
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General Information
Symbol
Dmel\apblt
Species
D. melanogaster
Name
blot
FlyBase ID
FBal0000654
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Mutagen
Nature of the Allele
Progenitor genotype
Associated Insertion(s)
Cytology
Description

Insertion of a retrotransposable element of the blood-family within the ap-RXa wing enhancer. The insertion causes a four bp duplication (CTGA). Exact coordinates of the 4 bp duplication: 2R:5735176.0.5735179 (FlyBase Release FB2014_06).

Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

At 18[o]C, less than 30% of the wings of homozygous apblt flies display minor abnormalities. Most of them show disruption of the posterior crossvein. At 29[o]C, approximately 70% of the wings show abnormalities, mostly notching of the posterior wing margin and reduced wing size. In many of them, the posterior compartment is severely affected.

Homozygous males have a mating rate 1% of wild type levels, this rate increases with age. If allowed sufficient time with females progeny are produced. For mating success apblt is a hypomorph. Homozygous males had equivalent sex appeal as wild type flies. 2 day old hemizygous apblt males have a 10--60 fold higher sex appeal than hemizygous ap+ males.

Homozygous flies have misshapen and blistered wings and have only 62% female receptivity to mature male ap+ flies. The receptivity is lower for flies heterozygous with Df(2R)M41A4. Females are fertile but have a low courtship intensity.

Wings blistered, sometimes inflated and dark due to trapped hemolymph. Mirror-image duplication of posterior wing blade structures occurs (FBrf0004792; FBrf0033471). Wing venation may be disrupted. Portions of posterior wing compartment may be transformed into anterior compartment structures, an effect like that of en. Despite relatively mild adult phenotype, extensive cell death observed, localized to wing pouch of imaginal discs; associated with acid phosphatase and lysosomal activity (FBrf0040733). Clonal analysis revealed nonautonomous expression of phenotype. Heterozygotes with ap4 or ap56f and hemizygotes show blistering phenotype only (FBrf0033471). apblt/ap73n shows transformation phenotype and aldehyde oxidase histochemical staining of these wing discs is consistent with transformation (FBrf0038704). Adults long lived.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT suppressed by
Statement
Reference

apblt has phenotype, non-suppressible by su(Hw)2

Enhancer of
Statement
Reference

apblt/ap[+] is an enhancer of eye phenotype of Scer\GAL4GMR.PF, fruNP0021

Additional Comments
Genetic Interactions
Statement
Reference

An apblt background enhances the rough eye phenotype related to fru isoform B, found upon expression of fruNP0021 under the control of Scer\GAL4GMR.PF.

ctL32; su(Hw)e2 flies heterozygous for apblt display a weak ct wing phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer

Groscurth, 1st Feb. 1931.

Comments
Comments

Overlaps with wild-type and expressivity is variable.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (6)