UAS regulatory sequences drive expression of mCherry. A silencing cassette ("sponge") of 20 repetitive "mir-13b" complementary sequences separated by variable four-nucleotide linker sequences has been inserted into the mCherry 3' UTR. The construct was generated using the pWALIUM10-moe vector.

Designed to knock-down the endogenous activity of both mir-13b-1 and mir-13b-2.

Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.

UAS regulatory sequences drive expression of mCherry. A silencing cassette ("sponge") of 20 repetitive "mir-13b" complementary sequences separated by variable four-nucleotide linker sequences has been inserted into the mCherry 3' UTR. The construct was generated using the pWALIUM10-moe vector.

Designed to knock-down the endogenous activity of both mir-13b-1 and mir-13b-2.

Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.