UAS regulatory sequences drive expression of mCherry. A silencing cassette ("sponge") of 20 repetitive mir-2c complementary sequences separated by variable four-nucleotide linker sequences has been inserted into the mCherry 3' UTR. The construct was generated using the pWALIUM10-moe vector.
Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.