UASt regulatory sequences drive expression of a sensor that is composed of mCherry fused to pHluorinSE. The coding sequence is targeted to the membrane via the presence of an N-terminal Tag:Myr(Src64B) tag (the dual fluorophore faces the cytoplasm). The mCherry-pHluorinSE dual fluorophore can act as an acidification sensor in vivo as pHluorinSE is pH sensitive but mCherry is pH-resistant; red fluorescence indicates an acidic environment (pH < 6), while yellow fluorescence indicates a pH greater or equal to 7. In addition, the C-terminal pHluorinSE moiety is expected to be more exposed to proteases than mCherry, such that the dual fluorophore can also be used as a degradation sensor by observing fluorescence in fixed samples; yellow fluorescence indicates both fluorophores are intact, red fluorescence indicates degradation of the C-terminal pHluorinSE moiety.