FlyBase Recombinant Construct Report: P{10XStat92E-GFP}

General Information

Symbol

P{10XStat92E-GFP}

FlyBase ID

FBtp0036675

Feature type

transgenic_transposable_element

Component Allele(s)

Avic\GFP^Stat92E.10X

Also Known As

10XSTAT92E-GFP, 10XSTAT-GFP, STAT92E-GFP, STAT-GFP, 10xStatGFP, 10XSTAT::GFP, 10XSTAT-dGFP, 10x STAT-GFP

Size

Expression Data

GFP reporter

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Avic\GFP^Stat92E.10X

Transgenic product class

Component

Use(s)

Regulatory region(s)

Socs36E

Encoded product / tool

EGFP

green fluorescent protein

Description

Five tandem repeats of a 441bp genomic region from the first intron of Socs36E, which contains two potential Stat92E binding sites, are cloned upstream of a minimal heat shock promoter driving expression of EGFP.

(Bach et al., 2007)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^Stat92E.10X

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14

embryonic large intestine | restricted

(Wells et al., 2013)

second instar larval stage

wing disc

(Hatini et al., 2013)

third instar larval stage

wing disc | restricted

(Hatini et al., 2013, Wells et al., 2013)

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

proventriculus | restricted

Comment: foregut/midgut junction

(Park and Kwon, 2011)

Additional Information

Statement

Reference

AvicGFP^Stat92E.10X is expressed in drpr and repo positive glial cells and is up-regulated in response to axotomy.

(Doherty et al., 2014)

AvicGFP^Stat92E.10X reporter activity is highly asymmetric within the anterior of the stage 14 large intestine. Cells on the inside of the hindgut curve show higher pathway activity than cells at comparable distances from the Upd-expressing large intestine on the outside of the curve. This difference in reporter activity is significant and not a consequence of increased cell density. Asymmetry in reporter activity is mirrored by expression of the endogenous JAK/STAT pathway target gene Socs36E.

(Wells et al., 2013)

In second instar larvae, reporter expression is observed throughout the disc proper. By early third instar, expression is repressed in the distal pouch and proximal notum,conincident with nub and eyg expression in these regions.

(Hatini et al., 2013)

A subset of the dorsomedial neurosecretory cells labelled by AvicGFP^Stat92E.10X also express ScerGAL4^VGAT.PF.

(Rajan and Perrimon, 2012)

This GFP-reporter of JAK-STAT signaling is specifically expressed in the cells at the junction of the foregut and the midgut in third instar larvae.

(Park and Kwon, 2011)

Stat92E reporter is expressed in ISCs and enteroblasts.

(Liu et al., 2010)

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

26197

w¹¹¹⁸; P{10XStat92E-GFP}1

26198

w¹¹¹⁸; P{10XStat92E-GFP}2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (37)

Reported As

Symbol Synonym

10 x STAT-GFP

(Huang et al., 2014)

10*Stat92E-GFP

(Yang et al., 2019)

10X STAT-GFP

(Miao et al., 2022)

10X STAT-{GFP}

(Classen et al., 2009)

10X Stat92E-GFP

(Mathur et al., 2010)

10X stat92E-GFP

(Hatini et al., 2013)

10X-stat92E-GFP

(Wang and Huang, 2009)

10XSTAT-DGFP

(Jiang et al., 2011)

10XSTAT-GFP

(Hsi et al., 2023, Molina-Gil et al., 2023, Grifoni et al., 2015, Chabu and Xu, 2014, Osman et al., 2012, Yan et al., 2011, Wu et al., 2010)

10XSTAT-dGFP

(Ren et al., 2010)

10XSTAT92E-GFP

(Gervais et al., 2019, Wang et al., 2014, Zhang et al., 2013, Bach et al., 2007, Ekas et al., 2006)

10XSTAT92E-eGFP

(Buchon et al., 2009)

10XSTAT::GFP

(von Frieling et al., 2020, Rajan and Perrimon, 2012)

10XSTATGFP

(Penfield and Montell, 2023)

10XStat-GFP

(Gunawan et al., 2013)

10XStat92E-GFP

(Harsh et al., 2020, Doherty et al., 2014, Yoon et al., 2011)

10Xstat-GFP

(Wang and Spradling, 2020)

10x STAT-GFP

(Starz-Gaiano et al., 2008)

10xSTAT-GFP

(Ho et al., 2019, Thomas and Strutt, 2014, Wright et al., 2011, Ekas et al., 2010, Cronin et al., 2009)

10xSTAT92E-GFP

(Yamazoe et al., 2021, Fregoso Lomas et al., 2016, Baumgartner et al., 2013, Van De Bor et al., 2011, Flaherty et al., 2009)

10xSTATGFP

(Wells et al., 2013)

10xStat92E-GFP

(Nagai et al., 2023, Järvelä-Stölting et al., 2021, Pan and O'Connor, 2021, Harris et al., 2020, Pearce et al., 2015, Richter et al., 2011, Liu et al., 2009)

10xStatGFP

(Sinha et al., 2013)

10xstat-GFP^D

(Zhai et al., 2015)

10xstat92E-GFP

(Tettweiler et al., 2019)

10xstatGFP

(Sinha et al., 2013)

P{10XStat92E-GFP}

(FlyBase, 1992-)

STAT-GFP

(Buchon et al., 2010, Starz-Gaiano et al., 2008)

STAT-GFP^(BL#26198)

(Willecke et al., 2011)

STAT92-GFP

(Parkhitko et al., 2020)

STAT92E-GFP

(Kugler et al., 2011)

Socs36E-GFP

(Gunawan et al., 2013)

Stat92E-GFP

(Shen et al., 2022, Wu et al., 2022, Zhang et al., 2017, Singh et al., 2011, Mathur et al., 2010, Takashima et al., 2008)

stat-GFP

(Sinha et al., 2013)

stat92E-GFP

(Cronin et al., 2009)

stat92E-binding site-GFP

(Cronin et al., 2009)

statGFP

(Sinha et al., 2013)

Secondary FlyBase IDs

References (64)

Report Sections

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