FlyBase Recombinant Construct Report: P{UAS-EKO[+]}

General Information

Symbol

P{UAS-EKO⁺}

FlyBase ID

FBtp0014962

Feature type

transgenic_transposable_element

Component Allele(s)

Sh^{EKO.UAS.GFP(GL)}

Also Known As

UAS-EKO, EKO, UAS-EKO⁺

Size

Expression Data

Associated insertion(s)

3 available

Molecular map

Description and Uses

Construct components

Component allele Sh^{EKO.UAS.GFP(GL)}

Transgenic product class

inframe_deletion

(White et al., 2001)

missense_variant

(White et al., 2001)

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

GFP(GL)

green fluorescent protein

Description

UASt sequences drive expression of a mutated form of the 29-4 Sh in which the inactivation domain (amino acid residues 1-29) has been replaced with GFP(GL) and which contains a D316N mutation in the S3 transmembrane domain and a K374Q mutation in the S4 transmembrane domain.

(White et al., 2001)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Sh^{EKO.UAS.GFP(GL)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

40973

y¹ w^*; wg^Sp-1/CyO; P{UAS-EKO⁺}323

40974

y¹ w^*; P{UAS-EKO⁺}222a P{UAS-EKO⁺}222b

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

EKO

(Gajula Balija et al., 2011, Peabody et al., 2009, Duch et al., 2008, Joiner et al., 2006)

P{UAS-EKO⁺}

(White et al., 2001, FlyBase, 1996-)

UAS-EKO

(Alassaf and Rajan, 2023, Brent and Rajan, 2020, Feng et al., 2018, Ping et al., 2015, Freeman et al., 2010, Parisky et al., 2008, Peabody et al., 2008, Fergestad et al., 2006, Luan et al., 2006, Ishimoto et al., 2005, Rodriguez Moncalvo and Campos, 2005, Suster et al., 2004, Osterwalder et al., 2001)

UAS-EKO⁺

(Weiss et al., 2019, Mosca et al., 2005)

UAS-Eko

(Feng et al., 2018, Crocker et al., 2010)

UAS-EkoIII

(Crocker et al., 2010)

UAS-Sh[act]

(Hartwig et al., 2008)

UAS-eko

(Selcho et al., 2017)

Secondary FlyBase IDs

References (27)

Report Sections

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