FB2024_04 , released June 25, 2024
Recombinant Construct: P{Act5C-GAL4.A}
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General Information
Symbol
P{Act5C-GAL4.A}
FlyBase ID
FBtp0014238
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4Act5C.PI
Also Known As
A5CGAL
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4Act5C.PI
Transgenic product class
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
GAL4
Also carries
FRT
Description

The FRT cassette has been excised, allowing expression of GAL4 driver sequences under the control of the Act5C promoter.

(Ito et al., 1997)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism | ubiquitous
(Ito et al., 1997)
embryonic stage -- larval stage
organism | ubiquitous

Comment: diffuse

(Sage et al., 2008)
larval stage
organism | ubiquitous
(Lang et al., 2012)
adult stage
eye photoreceptor cell | subset
(Shimozono et al., 2019)
Additional Information
Statement
Reference

Designed for use in mosaic analyses; used to drive gene of interest in ScerFLP1-generated clones. Transiently generated during mosaic analyses; ubiquitous expression throughout region of the generated clone, but not in rest of body (cell autonomous). Stocks for mosaic analysis are attached to progenitor construct P{AyGAL4}; see ScerGAL4FRT.Act5C elsewhere in this table. Stocks in which the descendent construct has been stably incorporated are associated with this allele; ubiquitous embryonic expression is driven.

(FlyBase Curators, 2017)

Transiently generated during mosaic analyses; ubiquitous expression throughout region of the generated clone, but not in rest of body (cell autonomous).

(Ito et al., 1997)

Designed for use in mosaic analyses; used to drive gene of interest in ScerFLP1-generated clones.

(Ito et al., 1997)
 
Marker for
Reflects expression of
Act5C
(Lang et al., 2012, Ito et al., 1997)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
P{Act5C-GAL4}
(Ahmad and Henikoff, 2001)
descendant(s)
Comments

construct_comment: Derived from P{Act5C-GAL4}25 by subjecting to P-transposase mutagenesis and selecting for mini-white-; experimentally verified that activity of Scer\GAL4 is retained.

(Ahmad and Henikoff, 2001)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
A5CGAL
(Schneiderman et al., 2010, Ahmad and Henikoff, 2001)
A5CGAL4
(Schneiderman et al., 2010)
P{Act5C-GAL4.A}
(Sass et al., 2003, Ahmad and Henikoff, 2001, FlyBase, 1996-)
Secondary FlyBase IDs
    References (4)