A 4.1kb BglII genomic fragment containing the His2Av gene tagged at the 3' end of the open reading frame (ORF) with GFP(S65T). The last two codons of the His2Av ORF have been removed and replaced with an additional 5 codons before the 5' end of the GFP(S65T) ORF.
The w+* marker is flanked by an FRT-cassette so that it can be excised.