FB2024_03 , released June 25, 2024
Insertion: Dmel\P{GawB}spictNP2367
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General Information
Symbol
Dmel\P{GawB}spictNP2367
Species
D. melanogaster
Name
FlyBase ID
FBti0034805
Feature type
transposable_element_insertion_site
Also Known As
spict-gal4
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
spict
Viability / fertility
Associated allele(s)
Scer\GAL4NP2367, spictNP2367
Stock availability
2 publicly available
Genomic Location
Chromosomal Location
2L ( 33F3 )
Sequence Location
2L:12,704,740..12,704,740 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:12,704,740..12,704,740 [+]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
33F3 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG218635
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
spict
(Olson et al., 2019, FlyBase Curators, 2008-)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
larval central brain
(Olson et al., 2019)
mesothoracic tergum primordium
(Olson et al., 2019)
wing hinge primordium
(Olson et al., 2019)
wing pouch | restricted
(Olson et al., 2019)
embryonic/larval pericardial cell
(Olson et al., 2019)
embryonic/larval dorsal vessel
(Olson et al., 2019)
adult stage | male
cyst cell of testis
(Chiang et al., 2017)
Additional Information
Statement
Reference

During spermatogenesis, ScerGAL4NP2367 drives expression in differentiating cyst cells, but not in cyst stem cells.

(Chiang et al., 2017)
 
Marker for
Reflects expression of
spict
(Chiang et al., 2017)
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location 2L:12704739-12704740 refined by FlyBase alignment of dbGSS accession AG218635 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
2367
(Olson et al., 2019)
P{GawB}NP2367
(FlyBase, 1992-)
P{GawB}NP2367-3-1
(FlyBase, 2005, FlyBase, 1992-)
P{GawB}spictNP2367
SpictNP2367-Gal4
(Yuen et al., 2021)
spict-Gal4
(Yuen et al., 2021)
spict-gal4
(Chiang et al., 2017)
Name Synonyms
Secondary FlyBase IDs
    References (7)