FB2024_04 , released June 25, 2024
Insertion: Dmel\P{EPgy2}Sec20EY04120
Open Close
General Information
Symbol
Dmel\P{EPgy2}Sec20EY04120
Species
D. melanogaster
Name
FlyBase ID
FBti0027293
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{EPgy2}
LINE ID

EY04120

Affected gene(s)
Hpr1, Sec20
Viability / fertility
viable, fertile
Associated allele(s)
Hpr1EY04120, Sec20EY04120
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
3R ( 83C4 )
Sequence Location
3R:5,822,494..5,822,494 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{EPgy2}.EY
Description

Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

(Bellen et al., 2011)

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w+mC and the mini-yellow marker y+mDint2, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.

(Bellen et al., 2004)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:5,822,494..5,822,494 [-]
(Levis, 2016.10.18)
Orientation

-

(Levis, 2016.10.18)
Cytological location (computed by FlyBase)
83C4 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
1360
(Levis, 2016.10.18)
Comments concerning location

Insertion is within a copy of 1360 that is within the 3' UTR of one annotated transcript of the CG2023 gene (there is no copy of 1360 at this site in the reference genome strain). This is approximately 1kb upstream of Hpr1.

(Levis, 2016.10.18)
Sequence Data
Inserted Element
Construct
P{EPgy2}
Size (Kb)

10.908

Component Allele(s)
TrlBRcRa
w+mC
y+mDint2
Molecular map
Affected Gene(s)
Insertion may affect gene
Hpr1
(Levis, 2016.10.18, Schneiderman et al., 2010)
Sec20
(Levis, 2016.10.18)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

EY04120

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Linkouts
GDP-EY
  • EY04120
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
EY04120
(Schneiderman et al., 2010, Levis R., 2009.3.30, Gene Disruption Project members, 2001-)
P{EPgy2}CG2023EY04120
P{EPgy2}EY04120
(FlyBase, 1992-)
P{EPgy2}Hpr1EY04120
P{EPgy2}Sec20EY04120
Name Synonyms
Secondary FlyBase IDs
    References (8)