FlyBase Insertion Report: Dmel\P{lacW}Hr39[k13215]

General Information

Symbol

Dmel\P{lacW}Hr39^k13215

Species

D. melanogaster

Name

FlyBase ID

FBti0007257

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(2)k13215

Affected gene(s)

Ecol\lacZ, Hr39

Viability / fertility

viable

Associated allele(s)

Ecol\lacZ^Hr39-k13215, Hr39^k13215

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2L ( 39B4 )

Sequence Location

2L:21,238,567..21,253,553 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:21,238,567..21,253,553 [+]

(Horner and Thummel, 1997)

Orientation

Cytological location (computed by FlyBase)

39B4 (inferred by FlyBase from sequence location)

Cytological location (reported)

39C1-39C2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

39C1--39C3 (in situ hybridization reported)

(Horner and Thummel, 1997)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(BDGP Project Members, 1994-1999)

Hr39

(Allen and Spradling, 2008, Allen and Spradling, 2008, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Hr39-k13215

Phenotypic Class Phenotype Manifest in Detailed Description

Hr39^k13215

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k13215

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

(Gene Disruption Project members, 2001-)

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

Insertion separable from lethal phenotype induced on same chromosome.

(Horner and Thummel, 1997)

maps to large intron of Hr39

(Horner and Thummel, 1997)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

P{lacW}Hr39^k13215

(Horner and Thummel, 1997, FlyBase, 1992-)

P{lacW}k13215

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0010379

References (9)

Report Sections

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