Changes introduced in the promoter regions of distinct LINEs allows transcriptional activators to stimulate cryptic Inr modules. The response of different promoter constructs to the same enhancer is significantly influenced by the number, position and type of core elements present.
Isolation and characterisation of a member of the F-element family, Fex. Fex is a full-size element of 4,690bp and codes for two complete open reading frames (ORFs). Functional analysis of the 5' region identifies a cis-acting control region within ORF1 that could account for most aspects of the temporal and spatial expression pattern.
Full length transcripts originate from the 'in' promoter, found at the 5' end and originate from the antisense 'out' promoter which transcribes towards the 'in' promoter.
The Fin and Fout promoters are functionally dissected in cell culture assays of templates carrying base substitutions and/or deletions across the +1 to +245 region of element F12. Fin promoter is internal to the transcribed region and consists of a proximal and distal element. Fout is a TATA-less promoter that directs transcription from three nearby sites.
Protein and RNA analysis of F-element DNA segments fused to Ecol\CAT identifies two promoters that transcribe in opposite orientations, in (oriented inward) and out (oriented outward). The 'in' promoter drives the synthesis of transcripts that initiate around residue +6 and are directed toward the F-element. 'in' transcripts are internal to the transcribed region and probably control the formation of F-element transposition RNA intermediates and gene products. The 'out' promoter drives the synthesis of transcripts that initiate at nearby sites within the +92 to +102 interval. Deletions knocking out the 'in' promoter do not impair 'out' transcription and conversely deletions knocking out the 'out' promoter do not impair 'in' transcription.
F-elements encode an open reading frame (ORF) that encodes a protein exhibiting extensive homology to the reverse transcriptase-like domain of the potential product of the I-element. This observation suggests F-elements and I-elements are closely related and presumably are mobilised within the genome by a similar mechanism.
First described by Dawid et al. (FBrf0035944) as an element within a copy of the R1-element type I 28S rDNA insertion sequence. The complete base sequence of a 3.5 kilobase element, Fw, has been reported by Di Nocera and Casari (FBrf0047228).
Other Information
Etymology
External Crossreferences and Linkouts ( 25 )
Crossreferences
GenBank Nucleotide
-
A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Sense and antisense transcripts of F-element have been detected at different stages of development.
Changes introduced in the promoter regions of distinct LINEs allows transcriptional activators to stimulate cryptic Inr modules. The response of different promoter constructs to the same enhancer is significantly influenced by the number, position and type of core elements present.
F-element, I-element and Doc basal promoters share the same architecture and functional organisation.
F-elements are located in both euchromatin and heterochromatin.
One of a class of genes with TATA-less promoters that have a subset of the conserved DPE sequence.
Isolation and characterisation of a member of the F-element family, Fex. Fex is a full-size element of 4,690bp and codes for two complete open reading frames (ORFs). Functional analysis of the 5' region identifies a cis-acting control region within ORF1 that could account for most aspects of the temporal and spatial expression pattern.
The distribution of transposable elements within heterochromatin indicates that they are major structural components of the heterochromatin.
Full length transcripts originate from the 'in' promoter, found at the 5' end and originate from the antisense 'out' promoter which transcribes towards the 'in' promoter.
The Fin and Fout promoters are functionally dissected in cell culture assays of templates carrying base substitutions and/or deletions across the +1 to +245 region of element F12. Fin promoter is internal to the transcribed region and consists of a proximal and distal element. Fout is a TATA-less promoter that directs transcription from three nearby sites.
Protein and RNA analysis of F-element DNA segments fused to Ecol\CAT identifies two promoters that transcribe in opposite orientations, in (oriented inward) and out (oriented outward). The 'in' promoter drives the synthesis of transcripts that initiate around residue +6 and are directed toward the F-element. 'in' transcripts are internal to the transcribed region and probably control the formation of F-element transposition RNA intermediates and gene products. The 'out' promoter drives the synthesis of transcripts that initiate at nearby sites within the +92 to +102 interval. Deletions knocking out the 'in' promoter do not impair 'out' transcription and conversely deletions knocking out the 'out' promoter do not impair 'in' transcription.
A cDNA clone, F-id1, is homologous to the F-element and has been isolated from cDNA library prepared from imaginal discs.
F-elements encode an open reading frame (ORF) that encodes a protein exhibiting extensive homology to the reverse transcriptase-like domain of the potential product of the I-element. This observation suggests F-elements and I-elements are closely related and presumably are mobilised within the genome by a similar mechanism.
First described by Dawid et al. (FBrf0035944) as an element within a copy of the R1-element type I 28S rDNA insertion sequence. The complete base sequence of a 3.5 kilobase element, Fw, has been reported by Di Nocera and Casari (FBrf0047228).