The current GtRNAdb (Release 16, accessed January 2016) contains a total of 295 D. melanogaster nuclear tRNA genes, of which 288 encode tRNAs decoding the 20 standard amino acids; 1 encodes a selenocysteine tRNA; 2 encode tRNAs with undetermined or unknown isotypes (CR30253 and CR31905); and 4 are predicted pseudogenes (CR30449, CR32761, CR32518 and CR32034).