Date: Tue, 05 Dec 2006  14:55:04  -0500
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC219
Cc: Jill Gresens <jgresensXXXX>, Stacey Christensen <sjchristXXXX>, mdealXXXX, kaufmanXXXX
Isolation and characterization of Df(3L)BSC219
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC219 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d06673 and PBac{WH}f06950. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d06673/P{XP}f06950 males. The males were heat shocked 
as larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC219 from the segment of P{XP}d06673 
to the left of its FRT site and the segment of P{XP}f06950 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(3L)BSC219 predicted from the Release 4 genomic 
coordinates of the transposable element insertions sites using are 
66D4;66D6. Df(3L)BSC219 failed to complement foi[j8E8].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX