From FlyBase-error@XXXX Wed Jan 10 17:40:32 2001 Envelope-to: gm119@XXXX Delivery-date: Wed, 10 Jan 2001 17:40:32 \+0000 From: FlyBase-error@XXXX Date: Wed, 10 Jan 2001 09:40:21 \-0800 (PST) X-Authentication-Warning: hedgehog.lbl.gov: web set sender to FlyBase-error using \-f To: flybase-updates@XXXX Cc: dan.hultmark@XXXX Subject: FlyBase error report for CG4740 on Wed Jan 10 09:40:21 2001 Content-Length: 1920 Error report from Dan Hultmark (dan.hultmark@XXXX) Gene or accession: CG4740 Missed gene Comments: The sequence for the mRNA of this gene, 'AttC', Accession number AF322249, was published recently. It shows that there is a mistake in the splicing pattern of CG4740. The predicted intron 1 does not exist and intron 2 is incorrectly spliced. In addition, the allele sequenced in the genome project has an eight base pair deletion, compared to the allele in Canton-S, which disrupts the reading frame. Thus, the former allele does probably not produce a functional protein. For comparison, the complete Canton-S mRNA sequence is given here: 1 atcgtcagtc aacagtcagc tgcttagtca agtagatcga aaatcaacag attaatacag 61 caagatgagc aaaattgttc tcctaattgt ggtcatcgtt ggcgtacttg gctcccttgc 121 ggtggccttg ccccaacgtc cgtataccca gccactgatc tactatcctc ctccaccgac 181 gccacccaga atctacagag cacgtcgcca ggtgttgggt ggatcactca catccaatcc 241 gagtggcggt gccgatgccc gattggatct aagcaaggcc gttggaactc ccgatcacca 301 tgtaattgga caagtgttcg cagcgggcaa cacgcagacc aaaccggtat ccactcccgt 361 aaccagcggc gccaccctgg gctacaacaa tcatggacac ggcctggaac tgaccaaaac 421 ccatacgccc ggtgtgcggg atagcttcca gcaaacggcc accgccaatt tgttcaacaa 481 cggtgttcac aatctggatg ccaaggcatt tgcctcgcag aatcagcttg ccaatggctt 541 caagttcgat agaaatggag ctgcactgga ctactcccac gtcaaaggtc atggagctac 601 cctgacgcac gccaatattc ccggattggg taagcaattg gagctgggtg ggcgtgccaa 661 tctttggcag tcgcaggatc gcaacacaag gctagatctg ggcagcacgg catccaagtg 721 gacaagtgga cccttcaagg gccagaccga tctgggtgcc aatctgggac tctcgcacta 781 ctttggataa aactatattc ttaaaaatat tttacaataa ctaagtgtta ttcaaaatat 841 ttattgacat aaattcaaac gaattttgtt gcttatttca aatgtgttaa aaatatatga 901 cttaactcaa aaaaaaaaaa aaaaa Browser: Mozilla/4.5 (Macintosh; I; PPC) Accessed from: query.pl, /www/hedgehog_8002/htdocs
