Population cages of wild-type, fertilized females were maintained at 25oC. Embryos were collected on apple juice agar plates in 2 hr windows, aged at 25oC for the required time, then dechorionated. Early larval collections were obtained from 2 hr embryo collections that were appropriately aged. Later larval and pupal stages were collected directly from flasks at the indicated time points. Adult flies were collected either within 4 hr of eclosion, or as one week old flies. Samples were snap-frozen. For each time point, samples were collected in quadruplicate.