FB2024_03 , released April 23, 2024
Project: Furlong_ChIP-chip
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General Information
Name
Furlong_ChIP-chip
Species
D. melanogaster
Project type
genome binding
FlyBase ID
FBlc0003340
Parent Project
    Data Provider
    Title
    ChIP-chip identification of binding sites for transcription factors that regulate mesodermal development.
    Accessions
      Overview
      Description
      Project attributes
      Biosample type
      whole organism
      (Ciglar et al., 2014, Rembold et al., 2014, Junion et al., 2012, Zinzen et al., 2009)
      Assay type
      ChIP-chip
      (Ciglar et al., 2014, Rembold et al., 2014, Junion et al., 2012, Zinzen et al., 2009)
      Reagent type
      Result type
      binding site identification
      (Ciglar et al., 2014, Rembold et al., 2014, Junion et al., 2012, Zinzen et al., 2009)
      Key genes
      (bait_protein)
      GO term(s)
      SO term(s)
      TF_binding_site
      Details
      Sample preparation
      Protocol

      Chromatin was prepared from formaldehyde-fixed embryos as previously described (FBrf0202481).

      (Bonn et al., 2012)
      Mode of Assay

      Immunoprecipitated chromatin was analyzed by Affymetrix GeneChip Drosophila Tiling 1.0R array

      (Bonn et al., 2012)
      Data analysis

      Peaks were called with TileMap software (v1) after quantile normalization. For each enriched region, probe intensity peaks were identified as extrema on a smoothed curve of the log2-ratio signal. Finally, 200 bp regions centered on identified extremas were defined. Peak calls were lifted over from Dmel_Release_5 to Dmel_Release_6 using the NCBI Genome Remapping tool.

      (Bonn et al., 2012)
      Comments
      Files
      Additional Information
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Furlong_ChIP-chip
      Name Synonyms
      ChIP-chip identification of binding sites for transcription factors that regulate mesodermal development.
      (Bonn et al., 2012)
      Secondary FlyBase IDs
        References (6)