FB2024_04 , released June 25, 2024
Reagent: LD_pBS_cDNA
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General Information
Name
LD_pBS_cDNA
Species
D. melanogaster
Reagent type
cDNA library
FlyBase ID
FBlc0000012
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pBS vector.
Accessions
Overview
Description

Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

(Rubin et al., 2000)
Available from
Biosample Source
Overview
Strain
iso-1
(Rubin et al., 2000)
Stage
Sex
Tissue isolated
organism
(Rubin et al., 2000)
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation

Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

Biosamples used (1)
Biosample
Type
Title
BS_LD_cDNA
whole organism
D. melanogaster, iso-1 strain, embryo (0-22 hr), source for cDNA.
Reagent Details
Methods
poly(A) RNA isolation
(Rubin et al., 2000)
oligo-dT priming
(Rubin et al., 2000)
size fractionation
(Rubin et al., 2000)
Molecular Construct used
Protocol

RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

(Rubin et al., 2000)
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
13,843 Clone(s)
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Files
Additional Information
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
LD
(Rubin et al., 2000)
LD_pBS
(Rubin et al., 2000)
LD_pBS_cDNA
Name Synonyms
LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pBS vector.
(Rubin et al., 2000)
Secondary FlyBase IDs
    References (1)