FB2024_03 , released April 23, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Su(var)205, nbs
FlyBase ID
FBig0000120309
Interaction Network
Interactions Browser links
Su(var)205 network
nbs network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0244191-2.coIP.WB
Reference
Bosso et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

bait

FLAG tag

nbs 

Nbs

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Bosso et al., 2019)
FBrf0244191-4.PD.WB
Reference
Bosso et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

bait

GST tag

Su(var)205 
Su(var)205 
Su(var)205 
Su(var)205 
nbs 

Nbs

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(var)205 
CSD domain
sufficient binding region
aa 136-206

coordinates relative to Su(var)205-PA

Su(var)205 
CSD domain
necessary binding region
aa 142-206

coordinates relative to Su(var)205-PA

Su(var)205 
CSD domain residue 191
mutation disrupting interaction
aa 191

I191E, coordinates relative to Su(var)205-PA

Su(var)205 
CSD domain residue 200
mutation disrupting interaction
aa 200

W200A, coordinates relative to Su(var)205-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Bosso et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)