FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CycC, Cdk8
FlyBase ID
FBig0000119944
Interaction Network
Interactions Browser links
CycC network
Cdk8 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0191850-5.PD.A
Reference
Loncle et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycC 

CycC

bait

GST tag

Cdk8 

Cdk8

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Loncle et al., 2007)
FBrf0191850-8.Y2H
Reference
Loncle et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycC 

CycC

bait

fused to GAL4 DNA-binding domain

Cdk8 

Cdk8

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

(Loncle et al., 2007)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Loncle et al., 2007)
External Crossreferences and Linkouts ( 0 )
References (1)