FB2024_04 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pnut, sip1
FlyBase ID
FBig0000106211
Interaction Network
Interactions Browser links
pnut network
sip1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0144953-14.Y2H
Reference
Shih et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait

fused to LexA DNA-binding domain

pnut 
sip1 

Sip1

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnut 
C-terminal region
sufficient binding region
aa 407-539

coordinates relative to pnut-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Shih et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Shih et al., 2002)
FBrf0144953-3.Y2H
Reference
Shih et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait

fused to LexA DNA-binding domain

sip1 

Sip1

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Shih et al., 2002)

1 sip1 clone obtained in the pnut screen.

(Shih et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Shih et al., 2002)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)