Source was S2 cells; bait produced from transfected construct; prey produced from endogenous gene.
pds5 construct contains both Myc and HA tags. Only HA tags used for pulldown in this experiment.
Source was S2 cells; bait produced from transfected construct; prey produced from endogenous gene.
Spacer amino acids between B2 and B3 BRC domains.
Source was S2 cells; bait produced from transfected construct; prey produced from transfected construct.
Proper spacing of non-BRC domain amino acids between BRC domains B2 and B3 of Brca2 are necessary for interaction with pds5. Removing these spacer amino acids disrupts the interaction. Region B2B3 of Brca2, including two BRC domains and spacer amino acids, is both necessary and sufficient for interaction with pds5.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from recombinant fusion protein.
Animo acid mutation locations for Brca2 relative to FBpp0072209 in release 2016_02.
Source was S2 cells; bait produced from transfected construct; prey produced from endogenous gene.
